Industrial Biotransformations

-Galactosidase
Saccharomyces lactis
4) Process parameters
conversion: 70 – 81 %
reactor type: plug-flow reactor
reactor volume: typical size: 1,500 to 250,000 L
capacity: e.g. 8,000 L · d –1 (Central del Latte, Italy)
enzyme supplier: Snamprogretti, Italy, and others
company: Sumitomo Chemical Co., Japan; Snow Brand Milk Products Co., Ltd.,
Japan; Central del Latte, Italy; and others
5) Product application
● Lactose needs to be removed before consumption by babies and people (e.g. Asians and Italians)
who are not able to produce or do not have enough β-galactosidase activity. These people
are called lactose-intolerant.
6) Literature
EC 3.2.1.23
● Cheetham, P.S.J. (1994) Case studies in applied biocatalysis – from ideas to products, in:
Applied Biocatalysis (Cabral, J.M.S., Best, D., Boross, L., Tramper, H., eds.) pp. 47–108, Harwood
Academic Publishers, Chur
● Honda, Y., Kako, M., Abiko, K., Sogo, Y., (1993) Hydrolysis of lactose in milk, in: Industrial
Application of Immobilized Biocatalysts (Tanaka, A., Tosa, T., Kobayashi, T., eds.) pp. 109–129,
Marcel Dekker Inc., New York
● Marconi, W., Morisi, F., (1979) Industrial application of fiber-entrapped enzymes, in: Appl. Biochem.
Bioeng. 2, Enzyme Technology, (Wingard, L.B., Katchalski-Katzir, E., Goldstein, L., eds.)
pp. 219–258, Academic Press, New York
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