Industrial Biotransformations

Aminopeptidase
Pseudomonas putida
● The whole cell catalyst of Pseudomonas putida accepts a wide range of substrates:
HO
H3C H3C
methyl
phenyl methyl-phenyl ethyl-phenyl
HO
4-hydroxy-methyl-phenyl 4-hydroxy-phenyl
Fig. 3.4.11.1 – 3
H3C CH3
iso-propyl
● Addition of Mn 2+ to the purified enzyme (up to 20 mM) in a preincubation step result in a
12-fold increase in activity, whereas Cu 2+ and Ca 2+ inhibit the enzyme at a concentration of
1 mM.
● The next table shows the K M and V maxvalues for different substrates:
Fig. 3.4.11.1 – 4
ethyl
R
NH2
O
S
NH 2
2-thiophenyl
S
Cl
1-chloro-phenyl
propenyl
1-methylsulfanyl-ethyl
EC 3.4.11.1
naphthyl
S
1-methylsulfanyl-propyl
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