Industrial Biotransformations

Naphthalene dioxygenase
Pseudomonas putida
glucose
● Alternatively a toluene oxidase from Pseudomonas mendocina KR-I mutant strains can be used
for the oxidation of indole to indole-3-ol:
3) Flow scheme
Not published.
N
H
4) Process parameters
reactor type: batch
company: Genencor International, Inc., USA
5) Product application
OH
OH
N
H
1 = L-tryptophan
2 = 1H-indole
3 = 2,3-dihydro-1H-indole-2,3-diol
4 = 1H-indole-3-ol
5 = indigo
Fig. 1.13.11.11 – 4
Fig. 1.13.11.11 – 5
NH 2
COOH
L-1 2
spontaneous
3 4 5
1 = 1H-indole
2 = 1H-indole-3-ol
N
H
tryptophanase
EC 1.13.11.11
● Indigo is an important brilliant blue pigment used extensively in the dyeing of cotton and
woollen fabrics.
N
H
OH
air
N
H
O
N
H
naphthalene
dioxygenase
E1 E2
E
toluene
monooxygenase
+ 1 / 2 O 2
1 2
N
H
OH
H
N
O
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