Carbaryl, Carbofuran, and Methomyl - National Marine Fisheries ...

TEP) are contained in Appendix D-1. The technical a.i. EC50 for water flea (D. magna), based
on a single test, is 5.6 μg/L. No 95% confidence interval is given for the test. Data for several
formulations with 43.7-81.5% carbaryl are presented. EC50s for the formulations range from
4.3-13.0 μg/L. Data are also available to compare toxicity of the technical grade to formulations
(43.7-81.5%) for the estuarine mysid shrimp, M. bahia. No 95% confidence intervals are given.
Two technical a.i. LC50s were provided in the data, 5.7 μg/L and 6.7 μg/L. Tests with
formulations (n=3) resulted in LC50s of 9.3-20.2 μg/L. No chronic tests were described for any
formulations.
For carbaryl, acute LC50 formulation data are also given for two species of fish, rainbow trout
(O. mykiss, 44-81.5 % carbaryl) and bluegill sunfish (Lepomis macrochirus, 5-50% carbaryl).
No 95% confidence intervals are given. Although other fish data for the technical carbofuran are
provided, a same-species comparison of toxicity data is better than evaluating against a range of
species, as the various species will exhibit a range of sensitivity. A single a.i. LC50 (1,200 μg/L)
is given for rainbow trout. A total of four LC50s are given for formulations, ranging from 1,400-
4,500μg/L. Three a.i. LC50s for bluegill are given, ranging from 5,000-14,000 μg/L. The
bluegill LC50s (n=4) for formulations range from 9,800-290,000 μg/L. Based on these data, it
appears the formulations tested are relatively similar in toxicity to the a.i. on an acute lethality
basis (survival endpoint). No chronic tests, which evaluate reproduction and growth, were
described for any formulations.
The carbofuran BE (EPA 2004a) lists some formulation toxicity data for aquatic invertebrates,
referenced to the EFED Science Chapter and other data referenced to the EPA Acquire Database.
In some cases, the formulation data referenced to the Science Chapter provides percent a.i. and in
others it contains designations such as “5G” or “50 WP”. In cases where the percent a.i. is
reported as a number, we presume the toxicity value has been corrected to percent a.i. It is
unclear whether the others have been corrected, whether the data from the Acquire database has
been corrected, or the specific products with which the toxicity data are associated.
Toxicity data presented in the carbofuran Science Chapter (EPA 2005) were found in Appendix
H of Appendix 1 to the main document, titled Animal Toxicity Tests with DERs. Study data on
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