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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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9. Technical Notes<br />

In this section:<br />

�Materials List<br />

�Media<br />

Preparation<br />

�Specialized<br />

MicroPlates<br />

Media<br />

Inoculating<br />

Fluid<br />

Miscellaneous<br />

Supplies<br />

MicroPlates<br />

Technical Notes<br />

This section gives specific information regarding consumable materials<br />

you’ll need to perform microbial identifications, as well as instructions for<br />

how to prepare isolation media and inoculating fluid.<br />

Materials List<br />

Item Description<br />

BUG + B Growth medium for gram-negative and gram-positive bacteria<br />

BUG + M Growth medium for gram-positive spore-forming rods<br />

BUG Growth medium for agricultural bacteria<br />

BUA Growth medium for anaerobes<br />

BUY Growth medium for yeasts<br />

CHOC Growth medium for fastidious gram-negative bacteria<br />

2% ME Growth medium for filamentous fungi (and select yeast species)<br />

GN/GP-IF Inoculating fluid for gram-negative and gram-positive bacteria<br />

AN-IF Inoculating fluid for anaerobic bacteria<br />

FF-IF Inoculating fluid for filamentous fungi (and select yeast species)<br />

Sterile water Inoculating fluid for yeasts<br />

Salicylate Inoculating fluid additive for some microbes<br />

Thioglycolate Inoculating fluid additive for some microbes (available in droppers)<br />

Streakerz Sterile 6” pointed streaking sticks<br />

LongSwabs Sterile 7” cotton-tipped swabs<br />

Transfer pipettes Sterile 6” disposable pipettes, graduated tip, 5 ml<br />

Reagent reservoirs Sterile reservoirs<br />

Test tube rack Autoclavable, holds 40 tubes, 20 mm diameter<br />

Pipette tips Sterile, racked, for repeating multichannel pipettor<br />

Pipette tips, filtered Sterile, filtered, racked, for repeating multichannel pipettor<br />

MicroPlate lids Gamma irradiated MicroPlate lids<br />

GN2 MicroPlates MicroPlates for gram-negative bacteria<br />

GP2 MicroPlates MicroPlates for gram-positive bacteria<br />

AN MicroPlates MicroPlates for anaerobic bacteria<br />

YT MicroPlates MicroPlate for yeasts<br />

FF MicroPlates MicroPlates for filamentous fungi (and select yeast species)<br />

TABLE 9-1. CONSUMABLE MATERIALS AND EQUIPMENT<br />

<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Apr 07 Section 9 � Page 1

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