MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...
MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...
MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...
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9. Technical Notes<br />
In this section:<br />
�Materials List<br />
�Media<br />
Preparation<br />
�Specialized<br />
MicroPlates<br />
Media<br />
Inoculating<br />
Fluid<br />
Miscellaneous<br />
Supplies<br />
MicroPlates<br />
Technical Notes<br />
This section gives specific information regarding consumable materials<br />
you’ll need to perform microbial identifications, as well as instructions for<br />
how to prepare isolation media and inoculating fluid.<br />
Materials List<br />
Item Description<br />
BUG + B Growth medium for gram-negative and gram-positive bacteria<br />
BUG + M Growth medium for gram-positive spore-forming rods<br />
BUG Growth medium for agricultural bacteria<br />
BUA Growth medium for anaerobes<br />
BUY Growth medium for yeasts<br />
CHOC Growth medium for fastidious gram-negative bacteria<br />
2% ME Growth medium for filamentous fungi (and select yeast species)<br />
GN/GP-IF Inoculating fluid for gram-negative and gram-positive bacteria<br />
AN-IF Inoculating fluid for anaerobic bacteria<br />
FF-IF Inoculating fluid for filamentous fungi (and select yeast species)<br />
Sterile water Inoculating fluid for yeasts<br />
Salicylate Inoculating fluid additive for some microbes<br />
Thioglycolate Inoculating fluid additive for some microbes (available in droppers)<br />
Streakerz Sterile 6” pointed streaking sticks<br />
LongSwabs Sterile 7” cotton-tipped swabs<br />
Transfer pipettes Sterile 6” disposable pipettes, graduated tip, 5 ml<br />
Reagent reservoirs Sterile reservoirs<br />
Test tube rack Autoclavable, holds 40 tubes, 20 mm diameter<br />
Pipette tips Sterile, racked, for repeating multichannel pipettor<br />
Pipette tips, filtered Sterile, filtered, racked, for repeating multichannel pipettor<br />
MicroPlate lids Gamma irradiated MicroPlate lids<br />
GN2 MicroPlates MicroPlates for gram-negative bacteria<br />
GP2 MicroPlates MicroPlates for gram-positive bacteria<br />
AN MicroPlates MicroPlates for anaerobic bacteria<br />
YT MicroPlates MicroPlate for yeasts<br />
FF MicroPlates MicroPlates for filamentous fungi (and select yeast species)<br />
TABLE 9-1. CONSUMABLE MATERIALS AND EQUIPMENT<br />
<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Apr 07 Section 9 � Page 1