MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

6. Interpreting Results
In this section:
� Reading
Printouts
�Assessing the
Accuracy of your
ID
�Pinning Down
an Uncertain ID
�Species
Comparison
�Searching and
Assessing Species
Information
� Using
Macroscopic and
Microscopic
Pictures
�Using the
Other Row
� Accessing
Raw OD values
Interpreting Results
Check the bottom section of the Data window. The software identifies
your microbe in the form of a ranked list. Entry #1 is the best match
selected from the database. The other identifications are ranked in
descending order of pattern match. Scroll down to see the whole list.
CLOSE-UP OF RESULTS SECTION OF DATA WINDOW
The ID box
Final identification results are displayed in the ID box at the top of the
results area of the Data window. The software considers the
possibility that even the #1 ranked species may not be a good enough
match. In this case, you will see “No ID” in the ID box.
Reading Printouts
Every time you read a MicroPlate, the printer will issue a hard copy
report. The information on these reports varies, depending on whether
you are working in manual or reader mode. See Appendices for plate
data statistics (A-1), and sample printouts and explanation of entries
(A-5).
Assessing the Accuracy of Your ID
If the answer to the following three questions is yes, you can feel
confident that ID #1 is accurate.
• Are the top-ranked ID choices on the list all the same (or closely
related) genera?
• Check the SIM (similarity index value) rating of ID #1. Is it greater
that .5?
• Check the DIST (distance) rating of ID #1. Is it greater than two
distance points away from the 2 nd choice.
MicroStation System/MicroLog User Guide Apr 07 Section 6 � Page 1