MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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MicroStation System/MicroLog Overview The Identification Process Microbial identification involves five basic steps. These steps apply to all identifications. A small number of species have peculiarities that may require an extra step or special handling techniques. The Microbial Identification Process Step 1 Step 2 Step 3 Step 4 Step 5 Isolate a pure culture on Biolog media Do a Gram stain (or wet prep for FF) and determine testing protocol Prepare inoculum at specified cell density Inoculate and incubate MicroPlate Read MicroPlate and determine ID Section 3 � Page 2 MicroStation System/MicroLog User Guide Nov 07
Follow directions closely to obtain accurate results. Step 1: Isolate a pure culture on Biolog media MicroStation System/MicroLog Overview Isolating a pure culture is not always easy. For example: Bacteria often have sticky surfaces and cells sometimes stick together in clumps. As a first step to accurate microbe identification, streak agar plates using correct techniques to generate well isolated colonies. Always use Biologrecommended culture media and growth conditions. See Section 4 for full culturing and incubation instructions. Step 2: Do a Gram stain and determine testing protocol For bacteria, proper Gram stain technique and interpretation are the important second step in the ID process. See pages 4.2 and 10.1-10.2. For FF identification, use the wet prep test as necessary to differentiate yeasts from filamentous fungi. Step 3: Prepare inoculum at specified cell density Microbiologists are sometimes trained to prepare cell suspensions by judging cell density by eye. This practice will not yield accurate and reproducible results. Cell density determines oxygen concentration − a key parameter to control when testing microorganisms in MicroPlates. In addition, Biolog has carefully optimized the required inoculating fluids. Don’t deviate from Biolog’s inocula preparation directions See pages 4.7 - 4.10. Step 4: Inoculate and incubate MicroPlate Pipet the specified amount of cell suspension into the MicroPlate, put the lid on, and incubate under the same conditions of temperature and atmosphere used to culture the microorganism. Biolog MicroPlates do not need oil overlays or color-developing chemicals. Step 5: Read MicroPlate and determine ID After an appropriate incubation time, read MicroPlates either by eye or using the MicroStation Reader. In either case, the pattern formed in the wells is entered into the software, which searches the database and provides an identification in seconds. Easy-to-Use Software MicroStation/MicroLog provides an easy-to-follow visual software interface to lead you through the identification process. The software can MicroStation System/MicroLog User Guide Nov 07 Section 3 � Page 3
Page 1 and 2: MicroStation System MicroLog Versi
Page 3 and 4: LICENSE AGREEMENT Notice: This is a
Page 5 and 6: Table of Contents Section Title 1.
Page 7 and 8: 10. Frequently Asked Questions…
Page 9 and 10: Welcome Section 1 � Page 2 Connec
Page 11 and 12: 2. Installation and Registration In
Page 13 and 14: Installation and Registration The A
Page 15 and 16: 8. E-mail the User Key File to tech
Page 17: MicroStation System/MicroLog Overvi
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Page 29 and 30: Preparing Samples Preparing an inoc
Page 31 and 32: Preparing Samples Recognizing fasti
Page 33 and 34: Preparing Samples Media shorthand:
Page 35 and 36: Preparing Samples Don’t forget to
Page 37 and 38: Preparing Samples b. Use a sterile
Page 39 and 40: Preparing Samples aids in liquefyin
Page 41 and 42: Preparing Samples Caution! Pipet th
Page 43 and 44: Preparing Samples Special Procedure
Page 45 and 46: Preparing Samples Initial culture m
Page 47 and 48: Reading MicroPlates Set Up tab Sele
Page 49 and 50: Reading MicroPlates When you’re e
Page 51 and 52: Reading MicroPlates 2. Enter reacti
Page 53 and 54: Reading MicroPlates Entering sample
Page 55 and 56: Reading MicroPlates Select file nam
Page 57 and 58: Reading MicroPlates Naming Workshee
Page 59 and 60: Reading MicroPlates This arrow show
Page 61 and 62: Reading MicroPlates Using Worksheet
Page 63 and 64: Reading MicroPlates 4. Click Save t
Page 65 and 66: Reading MicroPlates Wells with high
Page 67 and 68: Reading MicroPlates Exiting When yo
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Interpreting Results Understanding
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Interpreting Results Mismatch Type
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Interpreting Results What are those
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Interpreting Results NOTE: A1 value
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Interpreting Results Slide the scro
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Interpreting Results VS. CURRENT MI
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Interpreting Results “Other” fi
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Data Management and Compiling Datab
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Combining Data Files Data Managemen
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OK, Out, Hold, Atypical Sorting lab
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Remember! When building your own da
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DATABASE WINDOW Data Management and
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Arrow indicates selected entry Data
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Export Help Mark this choice if you
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8. Administration and Security In t
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Important! • If you test Password
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Log-In Privileges Administration an
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Remember that the password must be
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9. Technical Notes In this section:
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Making Biolog Universal Growth Agar
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� � � � Technical Notes Gen
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10. Frequently Asked Questions In t
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Q A Q A Q A Frequently Asked Questi
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Troubleshooting Symptom Cause Solut
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Troubleshooting Preparing Inocula S
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Troubleshooting MicroStation Reader
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Glossary Dendrogram Cluster diagram
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Glossary Spore, fungal Sterile, fun
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Culture Media Atmosphere Temperatur
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Appendices: Notes Notes: Section 13
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Appendix 4: Database Species Lists
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Appendix 5: Program Printouts Appen
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Appendix 5: Program Printouts 1�
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Appendix 5: Program Printouts Funga
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Appendix 5: Program Printouts Print
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Appendix 6: Dangerous Pathogens (DP
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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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