MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Preparing Samples
b. Use a sterile swab (Biolog P/N 3023 or 3021) wet with the
thioglycolate solution to spread a thin film across the entire surface
of the BUG + M agar plate (Biolog P/N 71103).
c. Allow the thioglycolate to dry on the agar before streaking the sporeforming
gram-positive rod strain (takes about 5 minutes).
3. With a Biolog sterile Streakerz stick (Biolog P/N 3025 or 3026), touch
an isolated colony and transfer to the BUG + M + T agar media. Make a
plus sign (+) on the center of the agar media. See Figure 1.
Figure 1: Plus Format to Streak
A
a. If the organism is a fast grower and spreads, make a single very
thin plus sign (+). This will avoid overgrowth and sporulation.
b. If the organism is a very slow grower and does not spread then
make up to three (3) streaks in the shape of the letter N in each
direction of the plus. Leave a small distance between the three
streaks to promote vegetative growth on all three streaks. Prepare
multiple agar plates (2 to 3) to insure that enough cells are
available to prepare the inoculation suspension.
PLUS “+” STREAKING METHOD CELL GROWTH AFTER INCUBATION
Section 4 � Page 10 MicroStation System/MicroLog User Guide Apr 07
B
c. The goal of this streaking technique is to limit cell growth to one
(1) to three (3) lines so that the cells along the edges have a good
supply of nutrients. This keeps the cells in an active state and
decreases sporulation.
4. Incubate at 30°C for 16-20 hours; do not exceed 16 hours for fast-growing
strains.