MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

When you’re
interpreting
results:
1. Trust your
eye over the
reader.
2. When in
doubt,
repeat the
test, using
stringent
technique.
GN, GP, and AN databases
1. Perform a visual assessment by checking the A1 well.
Interpreting Results
• If it contains a significant purple color, this color may be
obscuring the pattern. Re-examine your general setup
procedures.
• The strain may need to be retested with thioglycolate added to
the inoculating fluid.
• With some rare gram positive species, salicylate must be added
(in addition to thioglycolate) to eliminate the false positive
purple color.
2. Check for borderline reactions. The system essentially disregards
these when making identifications.
• Review and verify your testing procedures. Did you use all
optimal conditions?
• If there are more than 15 borderline reactions, visually check
the MicroPlate. If you used the reader, compare how it read the
pattern in relation to how you read it by eye. If you disagree
with the reader, click the Set Up tab on the menu bar. Select
Manual mode and correct reactions manually. Or you can use
the histogram function to adjust the thresholds (see Section 5).
Re-identify based on the modified pattern or incubate the
MicroPlate for a longer time (do not exceed time limits).
3. Check for a message in the ID box. When appropriate, the ID box
will offer additional information to guide your identification. For
example:
• ID of Salmonella, Shigella, or E. coli O157, ID box message
says “Confirm by Serology”
• ID of Listeria species or Neisseria gonorrhoeae, ID box
message says “Confirmation of ID is recommended.”
Species Comparison
MicroLog software has several features that can help with species
identification. You can compare any pattern with the expected pattern
of any species or taxa in the database. You can also display expected
species patterns visually.
To compare your #1 ID with others on the list of runners-up:
MicroStation System/MicroLog User Guide Apr 07 Section 6 � Page 5