MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Recommendations

Info

Sample Preparation Process For Filamentous Fungi Initial Culture Medium 2% ME 2% ME Wet Prep Results Hyphal elements Yeast cells Microbe Type Filamentous Fungi Yeast Culture Medium 2% ME 2% ME Temperature 26° C 26° C Atmosphere Air Air Culture Incubation Time 5 - 10 days 2 days Inoculating Fluid FF-IF FF-IF Inoculum Turbidity/ 75% T 75% T Turbidity Standard FF FF MicroPlate Type/µl per well FF FF 100 100 Incubation time (hours) 24, 48, 72, 96 24, 48, 72, 96 ABBREVIATIONS 2% ME = 2% Malt Extract Agar FF = Filamentous Fungi %T = percent transmittance IF = inoculating fluid Preparing Samples MicroStation System/MicroLog User Guide Apr 07 Section 4 � Page 17
Preparing Samples Initial culture medium Gram stain results Characterization test Characterization test Sample Preparation Process For Gram Negatives, Gram Positives, Anaerobes and Yeasts BUG + B* BUA + B BUY Gram stain and observe cell morphology Oxidase positive requires 30° C � Gram Negatives � � Gram Positives � Anaerobes � Kanamycin test for rapidly-growing, gramnegative rods Oxidase negative and TSI=K/K or K/A w Oxidase negative and TSI=A/A or K/A Requires CO2 or chocolate agar or < 1 mm colonies on BUG + B Microbe type GN-NENT GN-ENT GN-FAS GP-COCCUS-ROD, GP-COCCUS, GP-ROD Culture medium BUG + B or TSA + B BUG +B or TSA + B Temperature Typically 30º C Typically 35- 37º C GP-ROD SB (spore-forming bacillus) Chocolate BUG + B BUG + M + T (8drops in 3ml H2O) “+”streaking Atmosphere Air Air 6.5% CO 2 Air or 6.5% CO 2 (if required) Inoculating fluid Inoculum turbidity/ Turbidity standard GN/GP-IF (if A1 well is pos, add thioglycolate) 52% T GN-NENT GN/GP-IF + T (3 drops) 61% T GN-ENT Section 4 � Page 18 MicroStation System/MicroLog User Guide Apr 07 Yeasts � AN YT BUA + B BUY Typically 35-37º C Typically 35-37º C Typically 30º C Typically 35-37° C 26° C GN/GP-IF + T (3 drops) 20% T GP-COC & GP- ROD & GN-FAS GN/GP-IF + T (3 drops) (if A1 well is pos, add 1 ml salicylate) 20% T GP-COC & GP-ROD & GN-FAS Air ANA-H 2 free Air GN/GP-IF (NO THIO) 28% T GP-ROD SB AN-IF Water MicroPlate GN2 GN2 GN2 GP2 GP2 AN YT type/ µl per well 150 150 150 150 150 100 100 Incubation time (hours) 4-6, 16-24 4-6, 16-24 4-6, 16-24 4-6, 16-24 4-6, 16-24 20-24 24, 48, 72 *Note: Agricultural bacteria may be grown on BUG without blood.and at 30° C for enterics. ABBREVIATIONS TSI = Triple Sugar Iron Slant; A = Acid, A W = Weak Acid, K = Alkaline; BUG + B = Biolog Universal Growth Medium + blood; BUG + M = Biolog Universal Growth Medium + maltose; T = thioglycolate; %T = percent transmittance; GN-NENT = gram-negative non-enteric; GN-ENT = gram-negative enteric; GN-FAS = gram-negative fastidious; GP-COCCUS & GP-COC = gram-positive cocci; GP-ROD = gram-positive rod; GP-ROD SB = gram-positive rod, spore-forming bacillus; AN = anaerobe; YT = yeast; IF = inoculating fluid 65% T AN 47% T YT
Page 1 and 2: MicroStation System MicroLog Versi
Page 3 and 4: LICENSE AGREEMENT Notice: This is a
Page 5 and 6: Table of Contents Section Title 1.
Page 7 and 8: 10. Frequently Asked Questions…
Page 9 and 10: Welcome Section 1 � Page 2 Connec
Page 11 and 12: 2. Installation and Registration In
Page 13 and 14: Installation and Registration The A
Page 15 and 16: 8. E-mail the User Key File to tech
Page 17 and 18: MicroStation System/MicroLog Overvi
Page 19 and 20: Follow directions closely to obtain
Page 29 and 30: Preparing Samples Preparing an inoc
Page 31 and 32: Preparing Samples Recognizing fasti
Page 33 and 34: Preparing Samples Media shorthand:
Page 35 and 36: Preparing Samples Don’t forget to
Page 37 and 38: Preparing Samples b. Use a sterile
Page 39 and 40: Preparing Samples aids in liquefyin
Page 41 and 42: Preparing Samples Caution! Pipet th
Page 43: Preparing Samples Special Procedure
Page 47 and 48: Reading MicroPlates Set Up tab Sele
Page 49 and 50: Reading MicroPlates When you’re e
Page 51 and 52: Reading MicroPlates 2. Enter reacti
Page 53 and 54: Reading MicroPlates Entering sample
Page 55 and 56: Reading MicroPlates Select file nam
Page 57 and 58: Reading MicroPlates Naming Workshee
Page 59 and 60: Reading MicroPlates This arrow show
Page 61 and 62: Reading MicroPlates Using Worksheet
Page 63 and 64: Reading MicroPlates 4. Click Save t
Page 65 and 66: Reading MicroPlates Wells with high
Page 67 and 68: Reading MicroPlates Exiting When yo
Page 69 and 70: Interpreting Results Understanding
Page 71 and 72: Interpreting Results Mismatch Type
Page 73 and 74: Interpreting Results What are those
Page 75 and 76: Interpreting Results NOTE: A1 value
Page 77 and 78: Interpreting Results Slide the scro
Page 79 and 80: Interpreting Results VS. CURRENT MI
Page 81 and 82: Interpreting Results “Other” fi
Page 83 and 84: Data Management and Compiling Datab
Page 85 and 86: Combining Data Files Data Managemen
Page 87 and 88: OK, Out, Hold, Atypical Sorting lab
Page 89 and 90: Remember! When building your own da
Page 91 and 92: Data Management and Compiling Datab
Page 93 and 94: DATABASE WINDOW Data Management and
Page 95 and 96:
Data Management and Compiling Datab
Page 97 and 98:
Arrow indicates selected entry Data
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Export Help Mark this choice if you
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
8. Administration and Security In t
Page 111 and 112:
Important! • If you test Password
Page 113 and 114:
Log-In Privileges Administration an
Page 115 and 116:
Remember that the password must be
Page 117 and 118:
9. Technical Notes In this section:
Page 119 and 120:
Making Biolog Universal Growth Agar
Page 121 and 122:
� � � � Technical Notes Gen
Page 123 and 124:
10. Frequently Asked Questions In t
Page 125 and 126:
Q A Q A Q A Frequently Asked Questi
Page 127 and 128:
Troubleshooting Symptom Cause Solut
Page 129 and 130:
Troubleshooting Preparing Inocula S
Page 131 and 132:
Troubleshooting MicroStation Reader
Page 133 and 134:
Glossary Dendrogram Cluster diagram
Page 135 and 136:
Glossary Spore, fungal Sterile, fun
Page 137 and 138:
Culture Media Atmosphere Temperatur
Page 139 and 140:
Appendices: Notes Notes: Section 13
Page 141 and 142:
Appendix 4: Database Species Lists
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Appendix 5: Program Printouts Appen
Page 177 and 178:
Appendix 5: Program Printouts 1�
Page 179 and 180:
Appendix 5: Program Printouts Funga
Page 181 and 182:
Appendix 5: Program Printouts Print
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Appendix 6: Dangerous Pathogens (DP
show all

MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Create successful ePaper yourself

Delete template?

Save as template?