01.11.2012 Views

MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

Remember:<br />

Light purple<br />

reactions<br />

are<br />

considered<br />

positive as<br />

long as the<br />

color is<br />

noticeable<br />

when<br />

compared to<br />

the A1<br />

reference<br />

well.<br />

Reading MicroPlates<br />

• Click Read Next to sequentially read the next MicroPlate on<br />

the worksheet without returning to the Worksheet window.<br />

• A Confirmation window appears. Click OK if it displays the<br />

MicroPlate you wish to read.<br />

• The Manual Entry of Data window appears.<br />

• If necessary, click Cancel to return to the Data window.<br />

Assessing reactions<br />

Entering reactions in manual mode requires visual assessment of<br />

MicroPlate reactions. SEE Section 5, page 5 for more information on<br />

reading reactions manually.<br />

Table 5-1 will guide your visual assessment:<br />

Color Density How To Assess It<br />

Same as A1 well (usually colorless) Negative<br />

Noticeable purple color (more than A1 well) Positive<br />

Extremely faint color. Not sure Borderline<br />

Entering reactions<br />

TABLE 5-1. VISUALLY ASSESSING WELL COLOR DENSITY<br />

1. Enter reactions into the on-screen MicroPlate using any of the<br />

methods shown on page 5.6.<br />

2. Click OK.<br />

MANUAL ENTRY OF DATA WINDOW (TO ENTER PLATE REACTIONS)<br />

3. Click Print to print report (if you did not select Automatic<br />

Printout).<br />

<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Apr 07 Section 5 � Page 17

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!