MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Inoculating MicroPlates
Troubleshooting
Symptom Cause Solution
Pipetting problems Wells inoculated unevenly Dispense first aliquot from pipettor back into reservoir.
Depress lever smoothly and completely.
Make sure tips are lined up evenly and tightly seated.
Avoid pipetting or trapping air bubbles.
Visually check wells after inoculating.
Incorrect volumes used
Incubating MicroPlates
100 µl for anaerobic bacteria, yeasts and fungal.
150 µl for aerobic bacteria.
Symptom Cause Solution
Poor growth or poor
pattern formation in
MicroPlates
Wells at the four
corners or edges of
the MicroPlate have
lower liquid levels
after incubation
Wrong incubation
conditions
Wrong MicroPlate
Organism not in Database
Incubator is too dry
Filamentous fungi not
incubated in a closed
container
Incubate according to specified temperature and
conditions. See Section 4 and Appendices.
Incubate the MicroPlate at the same temperature as
the growth plate.
Make sure incubator humidity is sufficient.
Add a pan of water to humidify incubator, or incubate
MicroPlates in a plastic container humidified with
moist paper towels.
Make sure you have the FF MicroPlates in a closed
container or in plastic bags on trays. Do not add
additional moisture when incubating filamentous
fungi.
MicroStation System/MicroLog User Guide Apr 07 Section 11 � Page 5