MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Culture Media
Atmosphere
Temperature
Inoculating Fluid
Inoculum %T
MicroPlate - µl
per well
Incubation Time
(hours)
Appendix 2: Release 4.2 Setup Flowchart
Oxi +
Appendix 2: Release 4.2 Setup Flowchart (non-FF)
Oxi –
GN
GN-NENT GN-ENT GN-FASb BUG+B
BUG+B
CHOC
AIR
Typically
30°C
GN/GP-IF f
52%T
GN2 – 150 µl
4-6, 16-24
30°C
TSI=K/K
or K/A w
TSI=A/A
or K/A
AIR
Typically
35°-37°C
GN/GP-IF+T
61%T
GN2 – 150 µl
4-6, 16-24
Determine Gram Stain Morphology
and Optimal Incubation Temperature a
and Atmosphere
If no
reactions
Aerobic Bacteria
6.5 % CO 2
Typically
35°-37°C
GN/GP-IF+T
20%T
GN2 – 150 µl
4-6, 16-24
MicroStation System/MicroLog User Guide Apr 07 Section 13 �Page 2
GP
GP-COCCUS GP-ROD
BUG+B c
AIR/6.5 % CO 2 d
Typically
35°-37°C
GN/GP-IF+T g
20%T
GP2 – 150 µl
4-6, 16-24
GP-ROD SB
BUG+M+T
(See 4.9-13 PLUS
streaking)
AIR
Typically
30°C
GN/GP-IF
28%T
GP2 – 150 µl
4-6, 16-24
Yeast
Anaerobic Bacteria
AN
BUA+B
a
With the exception of thermophiles, all species are either 26°C, 30° or 35°-37°C.
f
If control well (A1) is positive, add thioglycolate to inoculating fluid.
b
Requiring CHOC or CO2 for growth or forming