MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Technical Notes
Making inoculating fluid (FF-IF)
Biolog supplies pre-made inoculating fluid, with the following
composition:
• 0.03% Tween 40 (e.g., Sigma P1504)
• 0.25% Gellan Gum (e.g., Phytagel, Sigma P8169)
However, if you wish to prepare your own, using Biolog’s formula.
1. Heat to boiling 1 liter of H2O.
2. Add 2.5 grams Gellan Gum and 0.3 grams Tween 40. Stir.
3. Turn off the heat but continue stirring until all components are
completely dissolved and the solution becomes clear.
4. Dispense the volume appropriate to obtain 16 ml post-autoclaving into
20 x 150 mm tubes.
5. Sterilize by autoclaving at 15 pounds of pressure and 121° C for 30
minutes.
Specialized MicroPlates
Biolog makes a series of additional MicroPlates for special applications.
While there is currently no database for these, you can use them to create
your own database with the software.
Table 9-2 lists these specialized MicroPlates and their uses. Contact
Biolog or your Biolog Distribution Partner for more information.
Specialized MicroPlate Use
SF-N2 and SF-P2 MicroPlates For testing of Sporulating and Filamentous microorganisms (such as
actinomycetes and fungi)
MT2 MicroPlate Contains same nutrient base and color chemistry as GN MicroPlate, but
without carbon sources. Add your own carbon sources to study the
metabolic capabilities of any microbe of your choice.
EcoPlate Contains the same color chemistry as the GN2 plate, but has three sets of 31
the identical carbon sources.
Note: the A1 well is used as the threshold algorithm control. Running
different samples on one plate may affect the positive/negative/borderline
calls.
TABLE 9-2. BIOLOG SPECIALIZED MICROPLATES
Section 9 � Page 6 MicroStation System/MicroLog User Guide Apr 07