MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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Appendix 3: FF Setup Flowchart Appendix 3: FF Setup Flowchart Sample Preparation Process for FF MicroPlate Initial culture medium 2% ME Wet prep results Hyphal elements Yeast cells Microbe type Filamentous Fungi Yeast Culture medium 2% ME 2% ME Temperature 26° C 26° C Atmosphere Air Air Culture Incubation time 5 - 10 days 2 days Inoculating fluid FF-IF FF-IF Inoculum turbidity/ Turbidity standard MicroPlate type/ µl per well Incubation time (hours) 75% T 75% T FF FF FF FF 100 100 24, 48, 72, 96 24, 48, 72, 96 *Note: We recommend that filamentous fungi be manipulated in a biological safety cabinet. ABBREVIATIONS 2% ME = 2% Malt Extract Agar FF = Filamentous Fungi %T = percent transmittance IF = inoculating fluid MicroStation System/MicroLog User Guide Apr 07 Section 13 � Page 3
Appendices: Notes Notes: Section 13 � Page 4 - MicroStation System/MicroLog User Guide Apr 07
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MicroStation System MicroLog Versi
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LICENSE AGREEMENT Notice: This is a
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Table of Contents Section Title 1.
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10. Frequently Asked Questions…
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Welcome Section 1 � Page 2 Connec
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2. Installation and Registration In
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Installation and Registration The A
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8. E-mail the User Key File to tech
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MicroStation System/MicroLog Overvi
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Follow directions closely to obtain
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Preparing Samples Preparing an inoc
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Preparing Samples Recognizing fasti
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Preparing Samples Media shorthand:
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Preparing Samples Don’t forget to
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Preparing Samples b. Use a sterile
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Preparing Samples aids in liquefyin
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Preparing Samples Caution! Pipet th
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Preparing Samples Special Procedure
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Preparing Samples Initial culture m
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Reading MicroPlates Set Up tab Sele
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Reading MicroPlates When you’re e
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Reading MicroPlates 2. Enter reacti
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Reading MicroPlates Entering sample
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Reading MicroPlates Select file nam
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Reading MicroPlates Naming Workshee
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Reading MicroPlates This arrow show
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Reading MicroPlates Using Worksheet
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Reading MicroPlates 4. Click Save t
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Reading MicroPlates Wells with high
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Reading MicroPlates Exiting When yo
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Interpreting Results Understanding
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Interpreting Results Mismatch Type
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Interpreting Results What are those
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Interpreting Results NOTE: A1 value
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Interpreting Results Slide the scro
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Interpreting Results VS. CURRENT MI
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Interpreting Results “Other” fi
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Data Management and Compiling Datab
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Combining Data Files Data Managemen
Page 87 and 88: OK, Out, Hold, Atypical Sorting lab
Page 89 and 90: Remember! When building your own da
Page 91 and 92: Data Management and Compiling Datab
Page 93 and 94: DATABASE WINDOW Data Management and
Page 97 and 98: Arrow indicates selected entry Data
Page 103 and 104: Export Help Mark this choice if you
Page 109 and 110: 8. Administration and Security In t
Page 111 and 112: Important! • If you test Password
Page 113 and 114: Log-In Privileges Administration an
Page 115 and 116: Remember that the password must be
Page 117 and 118: 9. Technical Notes In this section:
Page 119 and 120: Making Biolog Universal Growth Agar
Page 121 and 122: � � � � Technical Notes Gen
Page 123 and 124: 10. Frequently Asked Questions In t
Page 125 and 126: Q A Q A Q A Frequently Asked Questi
Page 127 and 128: Troubleshooting Symptom Cause Solut
Page 129 and 130: Troubleshooting Preparing Inocula S
Page 131 and 132: Troubleshooting MicroStation Reader
Page 133 and 134: Glossary Dendrogram Cluster diagram
Page 135 and 136: Glossary Spore, fungal Sterile, fun
Page 137: Culture Media Atmosphere Temperatur
Page 141 and 142: Appendix 4: Database Species Lists
Page 175 and 176: Appendix 5: Program Printouts Appen
Page 177 and 178: Appendix 5: Program Printouts 1�
Page 179 and 180: Appendix 5: Program Printouts Funga
Page 181 and 182: Appendix 5: Program Printouts Print
Page 187 and 188: Appendix 6: Dangerous Pathogens (DP
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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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