MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Technical Notes
Making Biolog Universal Yeast Agar (BUY)
1. Mix the following in a 2-3 liter container:
• 60 grams BUY Agar
• 1,000 ml purified, distilled, or deionized water
2. Gently boil while stirring to dissolve the agar and other components.
3. Cool an aliquot and measure the pH. Adjust pH with NaOH or HCl to
achieve a final pH of 5.6 + 0.4 at 25° C.
4. Sterilize by autoclaving at 15 pounds of pressure and 121° C for 15
minutes.
5. Cool to 45-50° C.
6. Dispense into sterile petri dishes.
Making 2% Malt Extract Agar
1. Mix the following in a 2-3 liter container:
• 20 grams Oxoid Malt Extract (LP0039B)
• 18 grams Bacteriological Grade Agar
• 1,000 ml purified, distilled, or deionized water
2. Gently boil while stirring to dissolve the agar and other components.
3. Cool an aliquot and measure the pH. Adjust pH with NaOH or HCl to
achieve a final pH of 5.5 + 0.2 at 25° C.
4. Sterilize by autoclaving at 15 pounds of pressure and 121° C for 15
minutes.
5. Cool to 45-50° C.
6. Dispense into sterile petri dishes.
Section 9 � Page 4 MicroStation System/MicroLog User Guide Apr 07