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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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Appendix 6 Dangerous Pathogens (DP) Database<br />

Organism Special Procedures<br />

Francisella tularensis Set up as a Gram-negative Fastidious organism<br />

Do not add thioglycolate to inoculating fluid<br />

Yersinia pestis Set up as an Gram-negative Enteric organism<br />

Do not add thioglycolate to inoculating fluid<br />

Grow only on BUG + Blood (not TSA + Blood)<br />

Burkholderia mallei and<br />

Set up as a Gram-negative Non-Enteric organism<br />

Burkholderia pseudomallei<br />

Grow only on BUG + Blood (not TSA + Blood)<br />

Brucella mellitensis<br />

(Use this procedure for B. abortus, B.<br />

canis, B. suis and other species that have<br />

been reclassified as B. mellitensis.)<br />

TABLE 12-1: PROCEDURE EXCEPTIONS FOR DANGEROUS PATHOGENS<br />

Incubate at 30° C<br />

Set up as a Gram-negative Fastidious organism<br />

Do not add thioglycolate to inoculating fluid<br />

Multiple chocolate plates are needed to obtain proper inoculum<br />

<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Nov 07 Section 13 � Page 53

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