MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Frequently Asked Questions
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Why is there a defined range for the turbidity of inocula?
It is essential to prepare inocula in a consistent and precise manner. The
Biolog redox test chemistry is sensitive to oxygen concentration, which is
determined by cell density. Use Biolog turbidity standards and calibrate
your turbidimeter.
Are Biolog’s Turbidity standards comparable to McFarland standards?
Biolog’s Turbidity standards have different set points and are not
comparable to McFarland standards. Biolog’s standards are set for cell
densities at which the Biolog test performs optimally. Use of Biolog
standards is necessary to achieve consistent and accurate results.
Can I use one incubator for all my strains and set it at 32°-33°c instead of
having two incubators at 30° c and 35°-37° c respectively?
Organisms grow better depending on the temperature of their
environment. Biolog has chosen the temperatures at which the organisms
in out database grow optimally. Using different temperatures than those
recommended will decrease the performance of your Biolog ID system.
We read our MicroPlates visually. Occasionally we are unsure whether a
reaction is positive or negative. How should we enter those wells?
Light purple reactions are considered positive as long as the color is
noticeable when compared to the A1 reference well. However, if you’re
still unsure, enter these reactions as “borderline.” If, for example, you
enter 4 out of 95 tests as borderline, the software will ignore the 4
borderline tests and base its identification on the remaining 91 tests. This
will give you far more accurate results than guessing wrong about
reactions you’re not sure of.
Section 10 � Page 2 MicroStation System/MicroLog User Guide Apr 07