MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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13. Appendices In this section: �Plate Data Statistics � Setup Flowcharts � Database Species Lists and Their Characteristics � Program Printouts Plate Type GN, GP, MT or Eco: AN: YT or Other: FF: General Read Statistics -Dual wavelength color statistic (590, 750) -A1-zeroed OD 750 turbidity statistic -Progressive identification algorithm -0.75, 0.5 similarity cut-offs for 4-6 hour, and 24 hours -Dual wavelength color statistic (590, 750) -A1-zeroed OD 750 turbidity statistic -Progressive identification algorithm -0.5 similarity cut-offs for 20-24 hours -Single wavelength color statistic (590) -End Point identification algorithm -0.75, 0.5, 0.5 similarity cut-offs for 24 hours, 48 hours, and 72 hours -Dual wavelength color statistic (490, 750) -A1-zeroed OD 750 turbidity statistic -End-point identification algorithm -0.9, 0.7, 0.65, 0.6, 0.6 similarity cut-offs for 1,2,3,4, and 7 days (7 day reading is present, however not included in the identification procedure) Appendix 1: Plate Data Statistics Appendix 1: Data Statistics For Various Plate Types Minimum Number of positives to call an ID 4-6 hour 3 read 24 hour 3 read 20-24 hour 7 read 24 hour 3 read 48 hour 3 read 72 hour 3 read 24 hour 30 48 hour 25 72 hour 20 96 hour 15 168 hour 5 ID specific limitations: Maximum number of borderline Reactions 25 Borderline Reactions 25 Borderline Reactions 25 Borderline Reactions 50 Borderline Reactions MicroStation System/MicroLog User Guide Apr 07 Section 13 � Page 1
Culture Media Atmosphere Temperature Inoculating Fluid Inoculum %T MicroPlate - µl per well Incubation Time (hours) Appendix 2: Release 4.2 Setup Flowchart Oxi + Appendix 2: Release 4.2 Setup Flowchart (non-FF) Oxi – GN GN-NENT GN-ENT GN-FASb BUG+B BUG+B CHOC AIR Typically 30°C GN/GP-IF f 52%T GN2 – 150 µl 4-6, 16-24 30°C TSI=K/K or K/A w TSI=A/A or K/A AIR Typically 35°-37°C GN/GP-IF+T 61%T GN2 – 150 µl 4-6, 16-24 Determine Gram Stain Morphology and Optimal Incubation Temperature a and Atmosphere If no reactions Aerobic Bacteria 6.5 % CO 2 Typically 35°-37°C GN/GP-IF+T 20%T GN2 – 150 µl 4-6, 16-24 MicroStation System/MicroLog User Guide Apr 07 Section 13 �Page 2 GP GP-COCCUS GP-ROD BUG+B c AIR/6.5 % CO 2 d Typically 35°-37°C GN/GP-IF+T g 20%T GP2 – 150 µl 4-6, 16-24 GP-ROD SB BUG+M+T (See 4.9-13 PLUS streaking) AIR Typically 30°C GN/GP-IF 28%T GP2 – 150 µl 4-6, 16-24 Yeast Anaerobic Bacteria AN BUA+B a With the exception of thermophiles, all species are either 26°C, 30° or 35°-37°C. f If control well (A1) is positive, add thioglycolate to inoculating fluid. b Requiring CHOC or CO2 for growth or forming
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MicroStation System MicroLog Versi
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LICENSE AGREEMENT Notice: This is a
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Table of Contents Section Title 1.
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10. Frequently Asked Questions…
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Welcome Section 1 � Page 2 Connec
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2. Installation and Registration In
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Installation and Registration The A
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8. E-mail the User Key File to tech
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MicroStation System/MicroLog Overvi
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Follow directions closely to obtain
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Preparing Samples Preparing an inoc
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Preparing Samples Recognizing fasti
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Preparing Samples Media shorthand:
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Preparing Samples Don’t forget to
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Preparing Samples b. Use a sterile
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Preparing Samples aids in liquefyin
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Preparing Samples Caution! Pipet th
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Preparing Samples Special Procedure
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Preparing Samples Initial culture m
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Reading MicroPlates Set Up tab Sele
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Reading MicroPlates When you’re e
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Reading MicroPlates 2. Enter reacti
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Reading MicroPlates Entering sample
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Reading MicroPlates Select file nam
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Reading MicroPlates Naming Workshee
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Reading MicroPlates This arrow show
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Reading MicroPlates Using Worksheet
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Reading MicroPlates 4. Click Save t
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Reading MicroPlates Wells with high
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Reading MicroPlates Exiting When yo
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Interpreting Results Understanding
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Interpreting Results Mismatch Type
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Interpreting Results What are those
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Interpreting Results NOTE: A1 value
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Interpreting Results Slide the scro
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Interpreting Results VS. CURRENT MI
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Interpreting Results “Other” fi
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Data Management and Compiling Datab
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Page 89 and 90: Remember! When building your own da
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Page 93 and 94: DATABASE WINDOW Data Management and
Page 97 and 98: Arrow indicates selected entry Data
Page 103 and 104: Export Help Mark this choice if you
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Page 111 and 112: Important! • If you test Password
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Page 117 and 118: 9. Technical Notes In this section:
Page 119 and 120: Making Biolog Universal Growth Agar
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Page 127 and 128: Troubleshooting Symptom Cause Solut
Page 129 and 130: Troubleshooting Preparing Inocula S
Page 131 and 132: Troubleshooting MicroStation Reader
Page 133 and 134: Glossary Dendrogram Cluster diagram
Page 135: Glossary Spore, fungal Sterile, fun
Page 139 and 140: Appendices: Notes Notes: Section 13
Page 141 and 142: Appendix 4: Database Species Lists
Page 175 and 176: Appendix 5: Program Printouts Appen
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Page 179 and 180: Appendix 5: Program Printouts Funga
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Appendix 6: Dangerous Pathogens (DP
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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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