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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> Overview<br />

3. Enter your Username and Password. Click OK.<br />

4. You will now be Logged In, and the Welcome interface will appear.<br />

Timed Log Out<br />

The software program has a default setting to automatically Log Out if the<br />

program has not been used for 15 minutes. The Login Time Out screen<br />

will appear. Simply select the Click Here button to stop the Log Out<br />

process and continue using the program. Otherwise the program will log<br />

off, return to the Welcome window, and display a new Log In dialog box.<br />

Logging Out and Switching Users<br />

There are 3 ways to end your use of the program:<br />

1. Select the Exit Tab and then click the Exit button.<br />

2. Click on the Change Users button in the center of the Welcome<br />

window. The Password Dialog box will be displayed for the new user<br />

to enter their username and password.<br />

3. Click on the windows page “X” button.<br />

The Math Behind the Software<br />

<strong>MicroStation</strong>/<strong>MicroLog</strong> software uses extensive computer algorithms to<br />

take the information from the observed pattern and compare it to the<br />

database.<br />

In simple terms, the software rapidly compares the positive-negativeborderline<br />

color pattern in the MicroPlate to the species pattern in the<br />

appropriate database. The patterns that most closely match your microbe’s<br />

pattern are shown on the screen in ranked order. Before making a decision<br />

on the result, the software considers the possibility that even the firstranked<br />

choice may not be a good match. It looks to see whether the first<br />

choice match is really “close enough” to be acceptable. If not, a “No ID”<br />

designation will result.<br />

For GN, GP, and AN databases, <strong>MicroStation</strong>/<strong>MicroLog</strong> uses a newly<br />

developed pattern matching method called Progressive ID (PID). This<br />

method more accurately identifies species patterns by considering the<br />

progressive sequence in which purple wells are formed. Typically,<br />

microorganisms will use their favorite carbon sources most rapidly and<br />

completely, resulting in dark purple wells that form quickly. Lesspreferred<br />

carbon sources are consumed slowly or incompletely, resulting<br />

in slower-forming or lighter purple color. The extra information<br />

considered by the PID matching method brings a higher level of<br />

consistency and accuracy, representing another innovation in Biolog’s<br />

technology.<br />

<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Nov 07 Section 3 � Page 5

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