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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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Check your<br />

MicroPlate<br />

before<br />

loading.<br />

Make sure<br />

the reaction<br />

pattern is<br />

well<br />

developed.<br />

Reading MicroPlates<br />

Fusarium, Penicillium, Trichoderma). The other<br />

databases are intended for use by researchers working<br />

on specific genera, not for routine food and air isolates.<br />

-Strain Name, Number: If you have additional information,<br />

enter it in these optional fields.<br />

-Incubation Time: Use the drop-down list to select the hours<br />

that the MicroPlate has incubated.<br />

Note: A 240-hour incubation time is listed for the FF<br />

database, however, there is no database for 240 hours.<br />

That listing is a research tool.<br />

-Other: If you have additional information, enter it in this<br />

optional field (colony morphology, culture conditions, etc.).<br />

-Comment: An optional comment can be entered in this field.<br />

3. Wipe the bottom of the MicroPlate to remove condensation and<br />

fingerprints.<br />

4. Place the MicroPlate on the reader tray with the A1 well at the top<br />

left-hand corner.<br />

5. Gently push the MicroPlate down until it snaps into a level position<br />

in the reader tray.<br />

6. Remove the MicroPlate lid.<br />

Note: For FF, leave the lid on. Replace it with a new lid if<br />

condensation is present.<br />

7. Click Read Next on the Data window.<br />

8. The reader calibrates itself, pulls the MicroPlate inside, takes a<br />

reading, displays the result on the Data window, and ejects the<br />

MicroPlate. Briefly do a visual check of the reaction with the +/b/-<br />

called by the auto-thresholding function (see pg 5.20 if adjustment<br />

is required).<br />

<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Apr 07 Section 5 � Page 9

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