MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

On all progressive
data windows:
Click on a specific
well to select it.
Find well
information in
“Key” box.
Click “Set to
Current Data
Value” to return
all settings to
number of positive
reactions your
current
MicroPlate has.
Interpreting Results
Progressive data analysis
MicroLog software uses a new method of pattern matching called
Progressive ID (PID). The PID method takes into account the
progressive nature by which purple color forms in the wells of the
MicroPlate. What typically happens after cells are inoculated is that
some wells turn purple quickly and strongly, resulting in dark purple
wells. These are usually the preferred carbon source for the microbe.
After additional incubation, other wells also turn purple, sometimes
forming weaker purple wells.
For example, if a MicroPlate has 23 purple wells, PID will compare
the pattern to the other species in the database at their stage of
development when they also had 23 purple wells. Each species in the
database responds to the search, as if to say “This is my pattern when I
had 23 positive reactions.” PID finds the best match among the species
in the database. The mathematics of matching the species to the pattern
is otherwise identical to that used in the previous method of End-point
ID (EID).
Reaction range
This display shows the entire metabolic progression of the species in a
single image. The boxes are entirely purple when the well is positive
100% of the time. A teal color indicates that the well is positive from
20% to 80% of the time. Empty boxes indicate that the substrate is
used < 20% of the time. Each box contains a black line that shows how
many positive reactions are on the given MicroPlate. This black line is
a gauge of progressive identification. The top right corner of the
window contains a key showing the current reaction of a specific well
and how often it is positive.
REACTION RANGE WINDOW
MicroStation System/MicroLog User Guide Apr 07 Section 6 � Page 9