MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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When you’re interpreting results: 1. Trust your eye over the reader. 2. When in doubt, repeat the test, using stringent technique. GN, GP, and AN databases 1. Perform a visual assessment by checking the A1 well. Interpreting Results • If it contains a significant purple color, this color may be obscuring the pattern. Re-examine your general setup procedures. • The strain may need to be retested with thioglycolate added to the inoculating fluid. • With some rare gram positive species, salicylate must be added (in addition to thioglycolate) to eliminate the false positive purple color. 2. Check for borderline reactions. The system essentially disregards these when making identifications. • Review and verify your testing procedures. Did you use all optimal conditions? • If there are more than 15 borderline reactions, visually check the MicroPlate. If you used the reader, compare how it read the pattern in relation to how you read it by eye. If you disagree with the reader, click the Set Up tab on the menu bar. Select Manual mode and correct reactions manually. Or you can use the histogram function to adjust the thresholds (see Section 5). Re-identify based on the modified pattern or incubate the MicroPlate for a longer time (do not exceed time limits). 3. Check for a message in the ID box. When appropriate, the ID box will offer additional information to guide your identification. For example: • ID of Salmonella, Shigella, or E. coli O157, ID box message says “Confirm by Serology” • ID of Listeria species or Neisseria gonorrhoeae, ID box message says “Confirmation of ID is recommended.” Species Comparison MicroLog software has several features that can help with species identification. You can compare any pattern with the expected pattern of any species or taxa in the database. You can also display expected species patterns visually. To compare your #1 ID with others on the list of runners-up: MicroStation System/MicroLog User Guide Apr 07 Section 6 � Page 5
Interpreting Results What are those + and – signs? These are signs showing mismatches between your sample and the database record. + > 80% pos 1. After a pattern is entered, the on-screen MicroPlate automatically displays the major mismatches between the #1 ranked species and your MicroPlate. 2. To compare your ID with other species on the list, click on the species you want to view. A small arrow appears next to the selected organism and the major mismatches are displayed. – < 20% pos Mismatches displayed PLUS (+) AND MINUS (–) MISMATCHES 3. To compare against a species that is not in the 10 top-ranking, click on the Other row. See page 13 Searching and Assessing Species Information MicroLog software gives you an opportunity to find out more about the range of reaction patterns of species in the MicroLog database (or any record in a user database). There are three possible ways to access the species information feature, all from the Data window: • Using the View Database button on the selection bar. • Using the ID box • Using the “Other” row Using the View Database Button You can get advanced species information without running identification, for single or multiple records in any database. Section 6 � Page 6 MicroStation System/MicroLog User Guide Apr 07
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MicroStation System MicroLog Versi
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LICENSE AGREEMENT Notice: This is a
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Table of Contents Section Title 1.
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10. Frequently Asked Questions…
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Welcome Section 1 � Page 2 Connec
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2. Installation and Registration In
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Installation and Registration The A
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8. E-mail the User Key File to tech
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MicroStation System/MicroLog Overvi
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Follow directions closely to obtain
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Page 29 and 30: Preparing Samples Preparing an inoc
Page 31 and 32: Preparing Samples Recognizing fasti
Page 33 and 34: Preparing Samples Media shorthand:
Page 35 and 36: Preparing Samples Don’t forget to
Page 37 and 38: Preparing Samples b. Use a sterile
Page 39 and 40: Preparing Samples aids in liquefyin
Page 41 and 42: Preparing Samples Caution! Pipet th
Page 43 and 44: Preparing Samples Special Procedure
Page 45 and 46: Preparing Samples Initial culture m
Page 47 and 48: Reading MicroPlates Set Up tab Sele
Page 49 and 50: Reading MicroPlates When you’re e
Page 51 and 52: Reading MicroPlates 2. Enter reacti
Page 53 and 54: Reading MicroPlates Entering sample
Page 55 and 56: Reading MicroPlates Select file nam
Page 57 and 58: Reading MicroPlates Naming Workshee
Page 59 and 60: Reading MicroPlates This arrow show
Page 61 and 62: Reading MicroPlates Using Worksheet
Page 63 and 64: Reading MicroPlates 4. Click Save t
Page 65 and 66: Reading MicroPlates Wells with high
Page 67 and 68: Reading MicroPlates Exiting When yo
Page 69 and 70: Interpreting Results Understanding
Page 71: Interpreting Results Mismatch Type
Page 75 and 76: Interpreting Results NOTE: A1 value
Page 77 and 78: Interpreting Results Slide the scro
Page 79 and 80: Interpreting Results VS. CURRENT MI
Page 81 and 82: Interpreting Results “Other” fi
Page 83 and 84: Data Management and Compiling Datab
Page 85 and 86: Combining Data Files Data Managemen
Page 87 and 88: OK, Out, Hold, Atypical Sorting lab
Page 89 and 90: Remember! When building your own da
Page 93 and 94: DATABASE WINDOW Data Management and
Page 97 and 98: Arrow indicates selected entry Data
Page 103 and 104: Export Help Mark this choice if you
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Page 111 and 112: Important! • If you test Password
Page 113 and 114: Log-In Privileges Administration an
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Page 117 and 118: 9. Technical Notes In this section:
Page 119 and 120: Making Biolog Universal Growth Agar
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10. Frequently Asked Questions In t
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Q A Q A Q A Frequently Asked Questi
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Troubleshooting Symptom Cause Solut
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Troubleshooting Preparing Inocula S
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Troubleshooting MicroStation Reader
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Glossary Dendrogram Cluster diagram
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Glossary Spore, fungal Sterile, fun
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Culture Media Atmosphere Temperatur
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Appendices: Notes Notes: Section 13
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Appendix 4: Database Species Lists
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Appendix 5: Program Printouts Appen
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Appendix 5: Program Printouts 1�
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Appendix 5: Program Printouts Funga
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Appendix 5: Program Printouts Print
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Appendix 6: Dangerous Pathogens (DP
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