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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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Frequently Asked Questions<br />

What should I do if I get different ID results at 4 hours and at 24 hours?<br />

This is most often the result of having a mixed culture. See Section 4 for<br />

recommended procedures and examine your culture carefully to see if it is<br />

mixed. Also, consider the similarity value at one incubation time vs. the<br />

other. Environmental strains will often grow a little differently than the<br />

lab strains used to make the databases. Some strains may have a high<br />

similarity in our 4 hour database, while others match our 24 hour database<br />

more closely. Compare you similarity values to see which is a better<br />

match.<br />

Are there any genera where the taxonomy is still undergoing change?<br />

Taxonomists have not yet agreed upon how all species should be<br />

delineated and microbiology is an ever-evolving science. We update and<br />

expand our software library on a continual basis, but at this point there are<br />

certain genera still undergoing revision. These include Bacillus,<br />

Corynebacterium, Enterobacter, Pseudomonas, Aeromonas, Vibrio,<br />

Acinetobacter, Moraxella, Clostridium, and Candida.<br />

www.dsmz.de/bactnom is a good site for current bacterial nomenclature.<br />

You can also link to this site through Biolog’s web page.<br />

www.cbs.knaw.nl is a good site for current yeast and filamentous fungi<br />

nomenclature and information.<br />

Our lab is interested in your quality control and validation procedures.<br />

Where do we get this information?<br />

Material Safety Data Sheets, Quality Control organism information, and<br />

Certificates of Performance are available on the website. Validation<br />

procedures, reagents kits and bacterial strains are available from Biolog.<br />

<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Apr 07 Section 10 � Page 3

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