MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Caution!
Manually adjusting
thresholds will
change SIM and
DIST values. These
changes override
automatic
thresholds. This is
an advanced
technique. Call
Biolog Technical
Service if you need
help.
Reading MicroPlates
• Click Read Next to sequentially read the next MicroPlate on
the worksheet without returning to the Worksheet window.
• A Confirmation window appears. Click OK if it displays the
MicroPlate you want to read. The reader calibrates itself,
pulls the MicroPlate inside, takes a reading, displays the
result on the Data window, and ejects the MicroPlate.
• If necessary, click Cancel to return to the Data window.
8. Click Print to print the report (if you did not select Automatic
Printout).
9. Click Save to save data (if you did not select Auto Write to Data
file.) A Confirmation window appears.
10. Repeat Steps 5-9 for all MicroPlates.
Adjusting Thresholds Manually
The software allows you to view histograms of MicroPlate patterns. In
most cases, use of this feature should not be necessary to obtain
accurate results. However, this advanced technique can be useful if
you feel that the automatic reading of your MicroPlate does not match
your visual interpretation. You can adjust either or both of the
thresholds to override the automatic reading and obtain what you feel
is the correct result. You can only adjust thresholds in Reader mode
and for only GN, GP and AN plates
1. Click on the small DW Data tab at the upper right edge of your
on-screen Microplate (on the Data window). You’ll see an onscreen
MicroPlate of your sample, showing optical densities.
2. Click the DW Histogram tab. A histogram of the current ID
selection appears.
3. The histogram is divided into three lateral sections, which
represent color distribution within the MicroPlate. Wells with low
and high color density are on the left and right sides of the
histogram. The middle section (between the two vertical lines)
represents wells with borderline reactions.
MicroStation System/MicroLog User Guide Apr 07 Section 5 � Page 19