MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...
MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...
MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...
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Appendix 3: FF Setup Flowchart<br />
Appendix 3: FF Setup Flowchart<br />
Sample Preparation Process for FF MicroPlate<br />
Initial culture medium 2% ME<br />
Wet prep results Hyphal elements Yeast cells<br />
Microbe type Filamentous Fungi Yeast<br />
Culture medium 2% ME 2% ME<br />
Temperature 26° C 26° C<br />
Atmosphere Air Air<br />
Culture Incubation time 5 - 10 days 2 days<br />
Inoculating fluid<br />
FF-IF FF-IF<br />
Inoculum turbidity/<br />
Turbidity standard<br />
MicroPlate type/<br />
µl per well<br />
Incubation time<br />
(hours)<br />
75% T<br />
75% T<br />
FF<br />
FF<br />
FF<br />
FF<br />
100<br />
100<br />
24, 48, 72, 96 24, 48, 72, 96<br />
*Note: We recommend that filamentous fungi be manipulated in a biological safety cabinet.<br />
ABBREVIATIONS<br />
2% ME = 2% Malt Extract Agar<br />
FF = Filamentous Fungi<br />
%T = percent transmittance<br />
IF = inoculating fluid<br />
<strong>MicroStation</strong> <strong>System</strong>/<strong>MicroLog</strong> User Guide Apr 07 Section 13 � Page 3