MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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Technical Notes Making inoculating fluid (FF-IF) Biolog supplies pre-made inoculating fluid, with the following composition: • 0.03% Tween 40 (e.g., Sigma P1504) • 0.25% Gellan Gum (e.g., Phytagel, Sigma P8169) However, if you wish to prepare your own, using Biolog’s formula. 1. Heat to boiling 1 liter of H2O. 2. Add 2.5 grams Gellan Gum and 0.3 grams Tween 40. Stir. 3. Turn off the heat but continue stirring until all components are completely dissolved and the solution becomes clear. 4. Dispense the volume appropriate to obtain 16 ml post-autoclaving into 20 x 150 mm tubes. 5. Sterilize by autoclaving at 15 pounds of pressure and 121° C for 30 minutes. Specialized MicroPlates Biolog makes a series of additional MicroPlates for special applications. While there is currently no database for these, you can use them to create your own database with the software. Table 9-2 lists these specialized MicroPlates and their uses. Contact Biolog or your Biolog Distribution Partner for more information. Specialized MicroPlate Use SF-N2 and SF-P2 MicroPlates For testing of Sporulating and Filamentous microorganisms (such as actinomycetes and fungi) MT2 MicroPlate Contains same nutrient base and color chemistry as GN MicroPlate, but without carbon sources. Add your own carbon sources to study the metabolic capabilities of any microbe of your choice. EcoPlate Contains the same color chemistry as the GN2 plate, but has three sets of 31 the identical carbon sources. Note: the A1 well is used as the threshold algorithm control. Running different samples on one plate may affect the positive/negative/borderline calls. TABLE 9-2. BIOLOG SPECIALIZED MICROPLATES Section 9 � Page 6 MicroStation System/MicroLog User Guide Apr 07
10. Frequently Asked Questions In this section: �Answers to Common Questions Q A Q A Q A How I should I store my MicroPlates? Frequently Asked Questions Unpack MicroPlates as soon as you receive them and keep them refrigerated until use. Biolog MicroPlates should be kept refrigerated (not frozen). MicroPlates are fairly stable at room temperature; we suggest refrigeration because they must be kept cold for maximum shelf life. At moderately warm temperatures, they slowly begin to deteriorate. Visually examine MicroPlates before using them. You may see faint yellow-brown or pink shades in wells when they are dry (this is OK). However, if wells show significant color immediately after inoculating the MicroPlate with the cell suspension, they have most likely been heat damaged. Do I have to handle frozen or lyophilized cultures in a special way? Yes. Subculture frozen or lyophilized cultures two to three times before testing. Our lab would really like to use our own media. We know that our strains will grow on it. Is that all right? No. However tempting it may be to use nonrecommended media, using anything other than Biolog-recommended media is a mistake. The carbon source tests in our MicroPlates are configured for precise metabolic reactions. The metabolic state of an organism has a profound influence on the resulting MicroPlate pattern that is the basis for identification. Many species will give fewer positive reactions when grown on nonrecommended media. Use Biolog-recommended media only! MicroStation System/MicroLog User Guide Apr 07 Section 10 � Page 1
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MicroStation System MicroLog Versi
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LICENSE AGREEMENT Notice: This is a
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Table of Contents Section Title 1.
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10. Frequently Asked Questions…
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Welcome Section 1 � Page 2 Connec
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2. Installation and Registration In
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Installation and Registration The A
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8. E-mail the User Key File to tech
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MicroStation System/MicroLog Overvi
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Follow directions closely to obtain
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Preparing Samples Preparing an inoc
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Preparing Samples Recognizing fasti
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Preparing Samples Media shorthand:
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Preparing Samples Don’t forget to
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Preparing Samples b. Use a sterile
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Preparing Samples aids in liquefyin
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Preparing Samples Caution! Pipet th
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Preparing Samples Special Procedure
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Preparing Samples Initial culture m
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Reading MicroPlates Set Up tab Sele
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Reading MicroPlates When you’re e
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Reading MicroPlates 2. Enter reacti
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Reading MicroPlates Entering sample
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Reading MicroPlates Select file nam
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Reading MicroPlates Naming Workshee
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Reading MicroPlates This arrow show
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Reading MicroPlates Using Worksheet
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Reading MicroPlates 4. Click Save t
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Reading MicroPlates Wells with high
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Reading MicroPlates Exiting When yo
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Interpreting Results Understanding
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Appendix 4: Database Species Lists
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Appendix 5: Program Printouts Appen
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Appendix 5: Program Printouts 1�
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Appendix 5: Program Printouts Funga
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Appendix 5: Program Printouts Print
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Appendix 6: Dangerous Pathogens (DP
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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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