MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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5. Reading MicroPlates………………………………………..………………. Logging In…………………………………………………………………………. Choosing Manual, Reader, or File Mode…………….……………………………. Choosing Printer Method………….……………………………………………….. Reading Plates Manually…………………………………………………………… Reading Plates Using the Plate Reader……………………………..……………… Reading from a Saved File………………………………………………….. Setting Up a Worksheet……………………………………………………………. Setting Up to Save Files……………………………………………………………. Using Worksheets to Read Multiple MicroPlates………………………….………. Adjusting Thresholds Manually……………………………………………………. Choosing a Database to Search…………………………………………….………. Exiting……………………………………………………………………………… 6. Interpreting Results…………………………………………………….…… Reading Printouts…………………………..……………………………………… Assessing the Accuracy of Your ID……………………………….………………. Pinning Down an Uncertain ID……………………………...………………….…. Species Comparison………….………………………………………………….…. Searching and Assessing Species Information….………………………………….. Using the View Database Button ……………….....………………………………. Using Macroscopic and Microscopic Pictures (FF)..………………………………. Using the “Other” Row…..……….………………………………………………... Assessing Raw OD Values…..…..………………………………………………… Assessing Data Values…..…..…………………………………………………….. 7. Data Management and Compiling Databases ..………………………… Backup Data File…………...………………….……………………………….….. Combining Data Files………..……………………….…………………………..... Viewing and Editing Files…..……………………………………………………... Compiling Data Files..……………...…………………………………………...…. Exporting ASCII Data………………………………………………………….…. Exporting to Microsoft Access……………………………………………………. Cluster Analysis……………………………..…………………………...…. 8. Administration and Security…………………...……….………………… What is Restricted Access Mode?………………………………………………... Administrator Options ………………………………………………..…………. Creating a User List ……………………………………………………………… Viewing the Log-In Log …………………………………………………………. Interpreting the Log-In Log…………………………………………………….… Changing or Lost Passwords …………………………………………..………… Unauthorized Log-In Attempts…………………………………………………… 9. Technical Notes…………………………………...……….………………… Materials List ……………………….……………………………………………... Media Preparation …………………………………………………………………. Specialized MicroPlates …………………………………………………………… 5.1 5.1 5.1 5.3 5.3 5.7 5.10 5.11 5.14 5.16 5.19 5.20 5.22 6.1 6.1 6.1 6.2 6.5 6.6 6.6 6.12 6.13 6.14 6.15 7.1 7.2 7.3 7.4 7.7 7.16 7.21 7.23 8.1 8.1 8.2 8.4 8.5 8.6 8.6 8.8 9.1 9.1 9.2 9.6
10. Frequently Asked Questions……………………………………………. 11. Troubleshooting……………………………………………………………. Gram Stain Identification……………………………...………………………....… Culturing Microorganisms…………………………………………………….…… Preparing Inocula…………………………………………………………………... Inoculating MicroPlates……………………………………………………………. Incubating MicroPlates…………………………………………………………….. <strong>MicroStation</strong> Reader…………..…………………………………..………………. 12. Glossary……………………………………………………………………… 13 Appendices..……..…………..………….………………………………..… Appendix 1: Data Statistics for Various Plate Types……………………………… Appendix 2: Release <strong>4.2</strong> Setup Flowchart…………………………………………. Appendix 3: FF Setup Flowchart………………………………………………….. Appendix 4: Database Species List and Their Characteristics……………………... Appendix 5: Program Printouts…………………………………………………….. Appendix 6: Dangerous Pathogen Database……………………………………….. 10.1 11.1 11.1 11.3 11.4 11.5 11.5 11.6 12.1 13.1 13.1 13.2 13.3 13.5 13.40 13.52
Page 1 and 2: MicroStation System MicroLog Versi
Page 3 and 4: LICENSE AGREEMENT Notice: This is a
Page 5: Table of Contents Section Title 1.
Page 9 and 10: Welcome Section 1 � Page 2 Connec
Page 11 and 12: 2. Installation and Registration In
Page 13 and 14: Installation and Registration The A
Page 15 and 16: 8. E-mail the User Key File to tech
Page 17 and 18: MicroStation System/MicroLog Overvi
Page 19 and 20: Follow directions closely to obtain
Page 29 and 30: Preparing Samples Preparing an inoc
Page 31 and 32: Preparing Samples Recognizing fasti
Page 33 and 34: Preparing Samples Media shorthand:
Page 35 and 36: Preparing Samples Don’t forget to
Page 37 and 38: Preparing Samples b. Use a sterile
Page 39 and 40: Preparing Samples aids in liquefyin
Page 41 and 42: Preparing Samples Caution! Pipet th
Page 43 and 44: Preparing Samples Special Procedure
Page 45 and 46: Preparing Samples Initial culture m
Page 47 and 48: Reading MicroPlates Set Up tab Sele
Page 49 and 50: Reading MicroPlates When you’re e
Page 51 and 52: Reading MicroPlates 2. Enter reacti
Page 53 and 54: Reading MicroPlates Entering sample
Page 55 and 56: Reading MicroPlates Select file nam
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Reading MicroPlates Naming Workshee
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Reading MicroPlates This arrow show
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Reading MicroPlates Using Worksheet
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Reading MicroPlates 4. Click Save t
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Reading MicroPlates Wells with high
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Reading MicroPlates Exiting When yo
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Interpreting Results Understanding
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Interpreting Results Mismatch Type
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Interpreting Results What are those
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Interpreting Results NOTE: A1 value
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Interpreting Results Slide the scro
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Interpreting Results VS. CURRENT MI
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Interpreting Results “Other” fi
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Data Management and Compiling Datab
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Combining Data Files Data Managemen
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OK, Out, Hold, Atypical Sorting lab
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Remember! When building your own da
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DATABASE WINDOW Data Management and
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Arrow indicates selected entry Data
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Export Help Mark this choice if you
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8. Administration and Security In t
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Important! • If you test Password
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Log-In Privileges Administration an
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Remember that the password must be
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9. Technical Notes In this section:
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Making Biolog Universal Growth Agar
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� � � � Technical Notes Gen
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10. Frequently Asked Questions In t
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Q A Q A Q A Frequently Asked Questi
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Troubleshooting Symptom Cause Solut
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Troubleshooting Preparing Inocula S
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Troubleshooting MicroStation Reader
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Glossary Dendrogram Cluster diagram
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Glossary Spore, fungal Sterile, fun
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Culture Media Atmosphere Temperatur
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Appendices: Notes Notes: Section 13
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Appendix 4: Database Species Lists
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Appendix 5: Program Printouts Appen
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Appendix 5: Program Printouts 1�
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Appendix 5: Program Printouts Funga
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Appendix 5: Program Printouts Print
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Appendix 6: Dangerous Pathogens (DP
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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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