MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Technical Notes
Caution!
If you’re making
your own media,
follow
instructions
exactly. Take
care to avoid
contamination.
Media Preparation
Biolog can provide you with all the pre-made media you will need for
microbe identification. However, you can also prepare your own media,
using Biolog’s formulas.
Making Biolog Universal Growth Agar + blood (BUG + B)
1. Mix the following in a 2-3 liter container:
• 57 grams BUG Agar
• 950 ml purified, distilled, or deionized water
2. Gently boil mixture while stirring to dissolve the agar and other
components.
3. Cool an aliquot and measure the pH. Adjust pH with NaOH or HCl to
achieve a final pH of 7.3 + 0.1 at 25° C.
4. Sterilize by autoclaving at 15 pounds of pressure and 121° C for 15
minutes.
5. Cool to 45-50° C.
6. Add 50 ml sterile fresh defibrinated sheep’s blood just prior to
dispensing and mix gently. (Use good quality blood with a hematocrit
of at least 40%).
7. Dispense into sterile petri dishes.
Making Biolog Universal Growth Agar + maltose (BUG + M)
1. Follow steps 1-5 above for BUG + B, except use 990 ml purified water.
2. Add 10 ml sterile solution of 25% maltose and mix thoroughly.
3. Dispense into sterile petri dishes.
Section 9 � Page 2 MicroStation System/MicroLog User Guide Apr 07