MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Recommendations

Info

Caution! Manually adjusting thresholds will change SIM and DIST values. These changes override automatic thresholds. This is an advanced technique. Call Biolog Technical Service if you need help. Reading MicroPlates • Click Read Next to sequentially read the next MicroPlate on the worksheet without returning to the Worksheet window. • A Confirmation window appears. Click OK if it displays the MicroPlate you want to read. The reader calibrates itself, pulls the MicroPlate inside, takes a reading, displays the result on the Data window, and ejects the MicroPlate. • If necessary, click Cancel to return to the Data window. 8. Click Print to print the report (if you did not select Automatic Printout). 9. Click Save to save data (if you did not select Auto Write to Data file.) A Confirmation window appears. 10. Repeat Steps 5-9 for all MicroPlates. Adjusting Thresholds Manually The software allows you to view histograms of MicroPlate patterns. In most cases, use of this feature should not be necessary to obtain accurate results. However, this advanced technique can be useful if you feel that the automatic reading of your MicroPlate does not match your visual interpretation. You can adjust either or both of the thresholds to override the automatic reading and obtain what you feel is the correct result. You can only adjust thresholds in Reader mode and for only GN, GP and AN plates 1. Click on the small DW Data tab at the upper right edge of your on-screen Microplate (on the Data window). You’ll see an onscreen MicroPlate of your sample, showing optical densities. 2. Click the DW Histogram tab. A histogram of the current ID selection appears. 3. The histogram is divided into three lateral sections, which represent color distribution within the MicroPlate. Wells with low and high color density are on the left and right sides of the histogram. The middle section (between the two vertical lines) represents wells with borderline reactions. MicroStation System/MicroLog User Guide Apr 07 Section 5 � Page 19
Reading MicroPlates Wells with high and low color density Wells with borderline color density HISTOGRAM (ON LEFT) AND OPTICAL DENSITIES (ON RIGHT) 4. Click the Manual radio button to make adjustments. 5. Use the horizontal scroll bars under the histogram to make adjustments to the thresholds. The top scroll bar adjusts the negative threshold (white line); the bottom scroll bar adjusts the positive threshold (red line). Green lines represent the automatic threshold levels. 6. Continue until you feel the adjusted threshold corresponds to your visual read of the MicroPlate. 7. Click Apply button to update thresholds. Note: FF MicroPlate types will have Pos/Neg, Data, and Histogram tabs for both color and turbidity. Choosing a Database to Search The software allows you to select which database you want to search. If you have created User (custom) databases you can search the MicroLog databases, the User database or both. For information on creating a User database, see Section 7. MicroLog databases may not be selected as a User database. Section 5 � Page 20 MicroStation System/MicroLog User Guide Apr 07 Click here to make adjustments
Page 1 and 2:
MicroStation System MicroLog Versi
Page 3 and 4:
LICENSE AGREEMENT Notice: This is a
Page 5 and 6:
Table of Contents Section Title 1.
Page 7 and 8:
10. Frequently Asked Questions…
Page 9 and 10:
Welcome Section 1 � Page 2 Connec
Page 11 and 12:
2. Installation and Registration In
Page 13 and 14: Installation and Registration The A
Page 15 and 16: 8. E-mail the User Key File to tech
Page 17 and 18: MicroStation System/MicroLog Overvi
Page 19 and 20: Follow directions closely to obtain
Page 29 and 30: Preparing Samples Preparing an inoc
Page 31 and 32: Preparing Samples Recognizing fasti
Page 33 and 34: Preparing Samples Media shorthand:
Page 35 and 36: Preparing Samples Don’t forget to
Page 37 and 38: Preparing Samples b. Use a sterile
Page 39 and 40: Preparing Samples aids in liquefyin
Page 41 and 42: Preparing Samples Caution! Pipet th
Page 43 and 44: Preparing Samples Special Procedure
Page 45 and 46: Preparing Samples Initial culture m
Page 47 and 48: Reading MicroPlates Set Up tab Sele
Page 49 and 50: Reading MicroPlates When you’re e
Page 51 and 52: Reading MicroPlates 2. Enter reacti
Page 53 and 54: Reading MicroPlates Entering sample
Page 55 and 56: Reading MicroPlates Select file nam
Page 57 and 58: Reading MicroPlates Naming Workshee
Page 59 and 60: Reading MicroPlates This arrow show
Page 61 and 62: Reading MicroPlates Using Worksheet
Page 63: Reading MicroPlates 4. Click Save t
Page 67 and 68: Reading MicroPlates Exiting When yo
Page 69 and 70: Interpreting Results Understanding
Page 71 and 72: Interpreting Results Mismatch Type
Page 73 and 74: Interpreting Results What are those
Page 75 and 76: Interpreting Results NOTE: A1 value
Page 77 and 78: Interpreting Results Slide the scro
Page 79 and 80: Interpreting Results VS. CURRENT MI
Page 81 and 82: Interpreting Results “Other” fi
Page 83 and 84: Data Management and Compiling Datab
Page 85 and 86: Combining Data Files Data Managemen
Page 87 and 88: OK, Out, Hold, Atypical Sorting lab
Page 89 and 90: Remember! When building your own da
Page 93 and 94: DATABASE WINDOW Data Management and
Page 97 and 98: Arrow indicates selected entry Data
Page 103 and 104: Export Help Mark this choice if you
Page 109 and 110: 8. Administration and Security In t
Page 111 and 112: Important! • If you test Password
Page 113 and 114: Log-In Privileges Administration an
Page 115 and 116:
Remember that the password must be
Page 117 and 118:
9. Technical Notes In this section:
Page 119 and 120:
Making Biolog Universal Growth Agar
Page 121 and 122:
� � � � Technical Notes Gen
Page 123 and 124:
10. Frequently Asked Questions In t
Page 125 and 126:
Q A Q A Q A Frequently Asked Questi
Page 127 and 128:
Troubleshooting Symptom Cause Solut
Page 129 and 130:
Troubleshooting Preparing Inocula S
Page 131 and 132:
Troubleshooting MicroStation Reader
Page 133 and 134:
Glossary Dendrogram Cluster diagram
Page 135 and 136:
Glossary Spore, fungal Sterile, fun
Page 137 and 138:
Culture Media Atmosphere Temperatur
Page 139 and 140:
Appendices: Notes Notes: Section 13
Page 141 and 142:
Appendix 4: Database Species Lists
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Appendix 5: Program Printouts Appen
Page 177 and 178:
Appendix 5: Program Printouts 1�
Page 179 and 180:
Appendix 5: Program Printouts Funga
Page 181 and 182:
Appendix 5: Program Printouts Print
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Appendix 6: Dangerous Pathogens (DP
show all

MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Create successful ePaper yourself

Delete template?

Save as template?