MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Reader and Saved File Modes Printout Key
Appendix 5: Program Printouts
1. Software version
2. If the printout is from a saved file, this entry lists file name, position of the entry in
the file, and edit status.
3. Threshold mode. This listing may say “Manual” or “Automatic.” In Automatic
mode, the software automatically calculates a threshold cutoff for negative and
positive reactions, indicated by numerical OD values. Any values between these two
numbers are considered borderline. The threshold can change from MicroPlate to
MicroPlate, depending on the intensity of the positive reactions and the clarity of the
negative reactions. In Manual Threshold mode, the user has changed the threshold
cutoffs to match the visual interpretation of the plate.
4. Time MicroPlate was read
5. Time file was modified
6. Does user have Unrestricted Access to software?
7. Number of MicroPlate
8. Sample identifiers
9. Operating mode (file)
10. Number of positive, borderline, and negative reactions
11. Name of database searched
12. Numbers seen in each well is the percent change data of the OD compared to well
A1 (A1 is always 0). DWD (dual wavelength data) = (590nm-750nm)x – (590nm-
750nm)A1 x 1000, where x = any well. Positive (), borderline ({}), and negative
reactions (no brackets). Numbers with no brackets and a plus (+) on the right side are
mismatches. The well read as negative, but at least 80% of the strains of that
organism tested in our database were positive for that well. Numbers with a < on the
left and a minus (–) on the right are also mismatches, but the well read as positive,
while the strains in our database were mostly negative for that well. Numbers with a
minus (-) to the left of the well value mean that the well’s OD was less than the OD
of the control well.
13. Species Iderntification/#1 identification
14. #2-9 identifications
MicroStation System/MicroLog User Guide Apr 07 Section 13 � Page 43