MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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Inoculating MicroPlates Troubleshooting Symptom Cause Solution Pipetting problems Wells inoculated unevenly Dispense first aliquot from pipettor back into reservoir. Depress lever smoothly and completely. Make sure tips are lined up evenly and tightly seated. Avoid pipetting or trapping air bubbles. Visually check wells after inoculating. Incorrect volumes used Incubating MicroPlates 100 µl for anaerobic bacteria, yeasts and fungal. 150 µl for aerobic bacteria. Symptom Cause Solution Poor growth or poor pattern formation in MicroPlates Wells at the four corners or edges of the MicroPlate have lower liquid levels after incubation Wrong incubation conditions Wrong MicroPlate Organism not in Database Incubator is too dry Filamentous fungi not incubated in a closed container Incubate according to specified temperature and conditions. See Section 4 and Appendices. Incubate the MicroPlate at the same temperature as the growth plate. Make sure incubator humidity is sufficient. Add a pan of water to humidify incubator, or incubate MicroPlates in a plastic container humidified with moist paper towels. Make sure you have the FF MicroPlates in a closed container or in plastic bags on trays. Do not add additional moisture when incubating filamentous fungi. MicroStation System/MicroLog User Guide Apr 07 Section 11 � Page 5
Troubleshooting MicroStation Reader Symptom Cause Solution Erratic or inaccurate reading Software won’t communicate with or initialize reader MicroPlate won’t go into reader Moisture, scratches, or smudges on MicroPlate Many borderline reactions on MicroPlate Remove MicroPlate lid before reading. Wipe bottom of MicroPlate before putting into reader. Review all sample preparation procedures for correctness and accuracy. Reading artifacts Always double-check results by eye. Verify that your visual reading coincides with reader results. Use the Histogram software function to manually adjust threshold if necessary. Wrong com port selected Choose correct com port. Loose cable connection Turn reader off. Unplug cable, then plug it back in. Turn reader on and try again. Unknown cause Turn reader off and exit program. Restart program, turn reader on, and try again. The reader has its own error messages, which should be self-explanatory. Call Biolog Technical Service if you need further assistance. Reader Bulb is Out/Low Use the System Check test in the Bio-Tek reader manual. (Use Air reference test in the Molecular Devices reader manual.) MicroPlate mispositioned Make sure MicroPlate snaps into place and is seated levelly. Make sure MicroPlate lid is off before reading. Remove MicroPlate and reposition in reader tray. Make sure A1 well is at the top left. Verify that software is communicating with reader. Section 11 � Page 6 MicroStation System/MicroLog User Guide Apr 07
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MicroStation System MicroLog Versi
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LICENSE AGREEMENT Notice: This is a
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Table of Contents Section Title 1.
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10. Frequently Asked Questions…
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Welcome Section 1 � Page 2 Connec
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2. Installation and Registration In
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Installation and Registration The A
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8. E-mail the User Key File to tech
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MicroStation System/MicroLog Overvi
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Follow directions closely to obtain
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Preparing Samples Preparing an inoc
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Preparing Samples Recognizing fasti
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Preparing Samples Media shorthand:
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Preparing Samples Don’t forget to
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Preparing Samples b. Use a sterile
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Preparing Samples aids in liquefyin
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Preparing Samples Caution! Pipet th
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Preparing Samples Special Procedure
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Preparing Samples Initial culture m
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Reading MicroPlates Set Up tab Sele
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Reading MicroPlates When you’re e
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Reading MicroPlates 2. Enter reacti
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Reading MicroPlates Entering sample
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Reading MicroPlates Select file nam
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Reading MicroPlates Naming Workshee
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Reading MicroPlates This arrow show
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Reading MicroPlates Using Worksheet
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Reading MicroPlates 4. Click Save t
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Reading MicroPlates Wells with high
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Reading MicroPlates Exiting When yo
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Interpreting Results Understanding
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Interpreting Results Mismatch Type
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Interpreting Results What are those
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Interpreting Results NOTE: A1 value
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Interpreting Results Slide the scro
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Page 83 and 84: Data Management and Compiling Datab
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Page 119 and 120: Making Biolog Universal Growth Agar
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Page 133 and 134: Glossary Dendrogram Cluster diagram
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Page 139 and 140: Appendices: Notes Notes: Section 13
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Appendix 5: Program Printouts Print
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Appendix 6: Dangerous Pathogens (DP
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MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

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