MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Follow
directions
closely to
obtain accurate
results.
Step 1: Isolate a pure culture on Biolog media
MicroStation System/MicroLog Overview
Isolating a pure culture is not always easy. For example: Bacteria often
have sticky surfaces and cells sometimes stick together in clumps. As a
first step to accurate microbe identification, streak agar plates using
correct techniques to generate well isolated colonies. Always use Biologrecommended
culture media and growth conditions. See Section 4 for full
culturing and incubation instructions.
Step 2: Do a Gram stain and determine testing protocol
For bacteria, proper Gram stain technique and interpretation are the
important second step in the ID process. See pages 4.2 and 10.1-10.2. For
FF identification, use the wet prep test as necessary to differentiate yeasts
from filamentous fungi.
Step 3: Prepare inoculum at specified cell density
Microbiologists are sometimes trained to prepare cell suspensions by
judging cell density by eye. This practice will not yield accurate and
reproducible results. Cell density determines oxygen concentration − a key
parameter to control when testing microorganisms in MicroPlates. In
addition, Biolog has carefully optimized the required inoculating fluids.
Don’t deviate from Biolog’s inocula preparation directions See pages 4.7 -
4.10.
Step 4: Inoculate and incubate MicroPlate
Pipet the specified amount of cell suspension into the MicroPlate, put the
lid on, and incubate under the same conditions of temperature and
atmosphere used to culture the microorganism. Biolog MicroPlates do not
need oil overlays or color-developing chemicals.
Step 5: Read MicroPlate and determine ID
After an appropriate incubation time, read MicroPlates either by eye or
using the MicroStation Reader. In either case, the pattern formed in the
wells is entered into the software, which searches the database and
provides an identification in seconds.
Easy-to-Use Software
MicroStation/MicroLog provides an easy-to-follow visual software
interface to lead you through the identification process. The software can
MicroStation System/MicroLog User Guide Nov 07 Section 3 � Page 3