MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

Assess SIM for ID #1
Gram-negative aerobes
Gram-positive aerobes
What Should I Do
About “No
Identification”?
Check the
on-screen + and –
signs for that
MicroPlate. You
can use these
mismatch
indicators to
assess what
happened.
Interpreting Results
1. The chart below will help you clarify your identification by using
SIM and DIST values:
USING SIM AND DIST TO ASSESS IDENTIFICATIONS
� must be > 0.5 at 16-24 hours incubation
� must be > 0.75 at 4-6 hours incubation
Anaerobic microbes � must be > 0.5 at 20-24 hours incubation
Yeasts � must be > 0.75 at 24 hours incubation
� must be > 0.5 at 48 or 72 hours incubation
Filamentous Fungi (& select yeasts) � must be ≥ 0.90 at 24 hours incubation
� must be ≥ 0.70 at 48 hours incubation
� must be ≥ 0.65 at 72 hours incubation
� must be ≥ 0.60 at 96 hours incubation
Assess SIM for top IDs
If SIMs for top IDs are < the SIM required AND all belong to the same genus AND if their total is ≥ the SIM
required, you can be confident that the microbe is in that genus. MicroLog will give you a genus-level ID
rather than a species-level ID.
Assess DIST for top IDs
If SIM is OK, but DIST between ID#1 and ID#2 are
very close
If you remain unconfident of the ID, try to
differentiate organisms by
� Good match to both species (some are quite close)
� Gram stain, colony morphology, oxidase and/or
catalase tests, additional tests (see Bergey’s Manual)
2. If you get a “No Identification” result, the % Prob value will not be
displayed. When this happens, check for black + and – signs in the
on-screen MicroPlate.
• If there are both + and – random mismatches, this is probably a
true mismatch. If so, this is probably a species not in Biolog’s
database. You can add it to your own user-created database. See
Section 8.
• If the mismatches are all + or all –, you may have made a
testing error. Refer to Table 6-1, and see Section 10.
Table 6-1 will assist you in figuring out the cause of these mismatches:
MicroStation System/MicroLog User Guide Apr 07 Section 6 � Page 3