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Composition of tomatoes and tomato products in antioxidants (WG1) page 84
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Some specific columns were shown to separate efficiently and in a short time isomers
of lycopene and β-carotene in reverse HPLC conditions. Five cis-lycopene peaks were
detected in human serum using a 5-µm Suplex pKb 100 (4.6x250 mm) column (Fig 19) (Stahl
and Sies 1992). When monomeric were changed for polymeric C18 columns (Vydac
201TP54) lutein was separated from zeaxanthin and some cis-isomers of β−carotene could be
detected (Fig 20) (Scott 1992).
Fig 19. Plasma carotenoids eluted on a Suplex pKb100 column
(From Stahl ans Sies 1992)