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Composition of tomatoes and tomato products in antioxidants (WG1) page 89<br />

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d) the solutions diluted are not stable overnight even in the refrigerator (should be<br />

kept in the freezer).<br />

9.1.7 Differences Between our Analytical Method and One of Hart & Scott.<br />

- Quantity of tomato derivatives to weigh . Particular extraction for peels.<br />

- Use of THF instead of CH2Cl2 to redilute samples after evaporation with rotavapor:<br />

THF seems to be the best solvent to extract lycopene in tomato matrix and secondary CH2Cl2<br />

is responsible of decreasing in lycopene concentration after storage (even just 1 day).<br />

- Temperature of columns: not thermostated at 22.5 (from 20 to 24°C air conditioned<br />

ambiant temperature.<br />

9.2 Vitamin C in tomatoes and in tomato products.<br />

Vitamin C, the antiscorbutic vitamin necessary for normal body functions, is correctly<br />

associated by consumers with tomato juice and other tomato products. Whole red-ripe<br />

tomatoes contain nearly all the vitamin C activity in the reduced ascorbic acid form.<br />

Dehydroascorbic acid has been reported to be from 1 to 5% of the total ascorbic acid in<br />

tomatoes.<br />

The ascorbic acid concentration in fresh ripe tomatoes is about 25 mg per 100g.<br />

9.2.1 Processing effects on Vitamin C.<br />

The maintenance of high levels of ascorbic acid products during processing has<br />

received considerable emphasis by food technologists. In the manufacture of tomato juice,<br />

ascorbic acid is destroyed, mainly by oxidation. Ascorbic acid is oxidized to dehydroascorbic<br />

acid, wich is further oxidized to degradation products with no vitamin C activity.<br />

The oxidation may be enzymatic or non enzymatic, and is catalized by copper ions.<br />

The rate of oxidation is dependent on the dissolved oxygen, enzyme content, dissolved

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