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Processing and Bioavailability (WG2) page 38<br />

__________________________________________________________________________________________<br />

14 Freezing O<br />

Survey of references<br />

Urbany and Horty (1989) evaluated colour and carotenoid changes during frozen storage of<br />

quick-frozen tomato cubes. Total carotenoids (determined by solvent extraction, column<br />

chromatography on magnesium oxide and spectrophotometric evaluation) decreased by<br />

approximately 40% during 6 months' storage at -20°C. Lycopene degradation was almost<br />

linear in time. Residual lycopene concentrations in 3 tomato varieties ranged from 75% to<br />

12% after 1 year's storage. Colour intensity was linearly correlated to residual carotenoid<br />

concentration in the products.<br />

Labrador et al. (1998) studied the effect of processing technique on the colour and<br />

lycopene content of tomato sauces for pizza during frozen storage. As stated above, sauces<br />

prepared in an open stirred kettle (o.s.k.) (15 min at 95 °C, in the open air) had significantly<br />

better colour attributes and lycopene content than those obtained in a tubular pasteuriser (105<br />

°C for an unspecified time adequate to reach an Fo value ensuring product stability) but<br />

showed a faster change in lycopene content during storage; this indicates lower stability (in<br />

the product obtained in o.s.k., the lycopene content diminishes from 110 to 16 ppm in 6<br />

months of storage at -10 °C, while for sauces obtained with the pasteuriser, the lycopene<br />

reduction is from 95 to 30 mg/kg).<br />

Comments and topics<br />

The scant bibliography on the subject makes it impossible to make any affirmations or to<br />

carry out an analysis of the hazards.<br />

Is this step an RCPL ?<br />

This aspect includes extremely varying aspects which cannot be grouped together, hence<br />

a hazard analysis would not appear to be applicable.<br />

.

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