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Composition of tomatoes and tomato products in antioxidants (WG1) page 87
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phase but if the injection volume exceeds 10-15 µl, then the individual peaks tended to
double. The best solution is probably to dissolve the dry residue with a mixture of methylene
chloride or THF with ethanol or methanol, and add mobile phase.
9. Description of specific methods for tomato antioxidant analysis
9.1 Lycopene analysis on tomato matrix
9.1.1. Fresh tomatoes
Ripe fruits (Quantity 3.5-5 Kg) are well washed and dried, crushed and cold
homogenized in an industrial cutter with variable speed.
All the system is maintained under vacumm; this is important to avoid dangerous air
soaks with subsequent oxidation.
The following phase is the analysis of the sample obtained right after homogenization
with pigment extraction. If it is impossible to analyze immediately, it is necessary to stock the
homogenized sample in under vacuum plastic bags hermetically sealed and placed in a light
air safe freezer as soon as possible.
9.1.2 Tomato Puree (light) (Passata di pomodoro) and tomato paste
Industrial tomato puree and tomato pastes have usually a high degree of homogeneity
and for this reason the product is analyzed without procedure.
9.1.3 Peeled tomatoes and crushed tomatoes
Before the extraction of pigments, canned peeled tomatoes and crushed tomatoes
(whole and pulp) are finely crushed with a hand crusher (homogenizer) and immediately
analyzed.