Abrir - Bienvenidos a la Biblioteca del INIFAP - Instituto ...
Abrir - Bienvenidos a la Biblioteca del INIFAP - Instituto ...
Abrir - Bienvenidos a la Biblioteca del INIFAP - Instituto ...
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Equipo:<br />
1. Micro-centrífuga.<br />
2. Centrífuga refrigerada de mesa.<br />
3. Vortex.<br />
4. Baño María a 45°C.<br />
5. Conge<strong>la</strong>dor a -20 °C ultra-conge<strong>la</strong>dor a -70 °C.<br />
6. Espectrofotrómetro.<br />
Literatura citada<br />
1. Ambion. (2007). "Phenols. Molecu<strong>la</strong>r Biology Grade." from<br />
http://www.ambion.com/catalog/CatNum.php?9720.<br />
2 Bartlett, J. M. S. (2003). Extraction of Nucleic Acid Temp<strong>la</strong>tes. PCR<br />
Protocols. J. M. S. Bartlett and D. Stirling. New Jersey, Humana Press<br />
Inc: 27-28.<br />
3 Bartlett, J. M. S. and A. White (2003). Extraction of DNA from Whole<br />
Blood. PCR Protocols. J. M. S. Bartlett and D. Stirling. New Jersey,<br />
Humana Press Inc: 29-31.<br />
4. Brayton, K. A., D. P. Knowles, et al. (2001). Efficient use of a small<br />
genome to generate antigenic diversity in tick-borne ehrlichial<br />
pathogens. Proc NatlAcad Sci U SA98(7): 4130-5.<br />
5. Bu<strong>del</strong>ier, K. and J. Schorr (1998). Purification of DNA by Anion-<br />
Exchange Chromatography. Current Protocols in Molecu<strong>la</strong>r Biology. F.<br />
M. Ausubel, R. Brent, R. E. Kingstonet al. USA, John Wiley & Sons. 1:<br />
2.1.11-2.1.18.<br />
6. Fahle, G. A. and S. H. Fischer (2000). Comparison of six commercial<br />
DNA extraction kits for recovery of cytomegalovirus DNA from spiked<br />
human specimens. J Clin Microbiol 38(10): 3860-3863.<br />
7. Figueroa, J. V., J. A. Alvarez, et al. (1996). Comparative sensitivity of<br />
two tests for the diagnosis of multiple hemoparasite infection of cattle.<br />
Ann N YAcad Sci 791: 117-27.<br />
8. Hernandez, R.,A. C. Chen, et al. (1998). Comparison of genomic DNA<br />
in various strains of Boophilus microplus (Acari: Ixodidae). J Med<br />
Entomol 35(5): 895-900.<br />
9. Kirby, K. S. (1957). A new method for the iso<strong>la</strong>tion of deoxyribonucleic<br />
acids; evidence on the nature of bonds between deoxyribonucleic acid<br />
and protein. Biochem J 66(3): 495-504.<br />
189
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