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Abrir - Bienvenidos a la Biblioteca del INIFAP - Instituto ...

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Mezc<strong>la</strong> maestra (master mix)<br />

La extracción de ADN se llevó a cabo por separado y, antes de preparar <strong>la</strong><br />

mezc<strong>la</strong> maestra, los primers y el ADN se mezc<strong>la</strong>ron en volumen proporcional,<br />

ajustando finalmente con agua, para tener un volumen final de 25 L.<br />

Literatura Citada.<br />

1. A<strong>la</strong> EL, P Brian JPhil, Bock R. 1997. PCR methods for the<br />

discrimination of Babesia bovis iso<strong>la</strong>tes. Vet Parasitol. 71(4): 223-<br />

237.<br />

2. Álvarez JA, AG Cantó. 1985. Epidemiología de <strong>la</strong> babesiosis. En:<br />

Parasitología. Vol. Conmemorativo de <strong>la</strong> Sociedad Mexicana de<br />

Parasitología. S.C. México, D.F. 55-72.<br />

3. Barros LS, Madruga RC, Araujo RF, Menk FC, Almeida OM, Melo PE,<br />

Kessler HR. 2005. Serological survey of Babesia bovis, Babesia<br />

bigemina and Anap<strong>la</strong>sma marginale antibodies in cattle from the<br />

semi-arid región of the state of Bahia, Brazil, by enzyme-linked<br />

immunosorbent assays. Mem Inst Oswaldo Cruz, Rio de Janeiro.<br />

100(6): 513-517.<br />

4. Bock RE, AJ De Vos. 2001. Immunity following use of Australian tick<br />

fever vaccine: a review of the evidence.Aust. Vet J. 79(12):832-839.<br />

5. Bono FM, Mangold JA, Baravalle EM, Valentini SB, Thompson SC,<br />

Wilkonsky ES, Echaide EI, Farber DM, Torioni MS. 2008. Efficiency of<br />

a recombinant MSA-2c-based ELISA to establish the persistence of<br />

antibodies in cattle vaccinated with Babesia bovis. Vet Parasitol.<br />

157(3.4): 203-210.<br />

195

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