Table of Contents - WOC 2012

WOC2012 Abstract Book
FP-RET-FR 60 (6)
To Evaluate the Safety and Outcomes of Vitreo-Retinal Interventions
Done for Retinal Complications in Patients of Treated
Retinoblastoma
Bhatia Kapil (1) , Babu Ajit (2) , Honavar Santos (3)
1. LV Prasad Eye Institute-Vitreo-Retina
2. LLV Prasad Eye Institute-Vitreo-Retina
3. LLV Prasad Eye Institute, Ocular Oncology and Oculoplastics
Purpose: To evaluate the safety and outcomes of vitreo-retinal interventions
done for retinal complications in Patients of treated retinoblastoma.
Methods: Retrospective interventional case series of 9 patients who underwent
vitreo-retinal interventions, in regressed retinoblastoma.
Results: mean age of presentation was 2 years (range 6months-5yrs). Mean
time interval between regression of retinoblastoma and intervention was 16.67
months (median 6 months, range 3 - 60 months). Pre-operative visual acuity
(BCVA) ranged from perception of light to 20/30. Only 2 patients had BCVA
? 20/60. Out of 9 patients, 6 underwent parsplana vitrectomy and 3 patients
underwent scleral buckling. Mean follow up time was 23.56 months (median 22
months, range 3 - 72 months). Six out of 9 patients had final BCVA of ? 20/60.
One patient developed vitreous hemorrhage after scleral buckling. None of
the patient had any evidence of systemic metastasis. One patient underwent
enucleation because of residual activity. There was no evidence of recurrence
in rest of the 8 patients.
Conclusion: VR interventions can have good outcomes in patients presenting
with retinal complications after retinoblastoma treatment. Chances of
metastasis and local recurrence are not high. However, a cautious approach
is warranted.
FP-RET-FR 60 (7)
The Inhibition Effect of Interfering RNA Target HIF-1? on VEGF
mRNA Expression in the Retina of Diabetic Rats
Moon Kun (1) , Meng Li-zhu (1)
1. Tianjin Eye Hospital
Objective: To evaluate the inhibitory effect of the hypoxia inducible factor-1,
HIF-1? specific siRNA on the expression of vascular endothelial growth factor
(VEGF) mRNA in retina issues in diabetic rat.
Methods: Randomized controlled trail was performed. Using pSilencer2.1-
U6neo for plasmid vector, HIF-1? specific siRNA recombinant plasmid was
constructed.
Results: HIF-1? siRNA recombinant plasmid was confirmed by enzyme
digestion and sequence analysis. FITC-Dextran fluorescent angiography
proved modeling construction successfully. String-of-beads, fluorescein
leakage and large nonperfusioncan be found in retinas of diabeitc rats.Realtime
RT-PCR revealed that the expression of VEGFmRNA was faint in the
normal group, but increased obviously in the DR group(P0.05). The
expression of VEGFmRNA in HIF-1? siRNA group were obviously decreased
compared with DR group. VEGFmRNA level was reduced by 32.76%, 43.60%,
47.70% and 50.86% at 24h, 48h, 72h, and 1w.
Conclusions: VEGFmRNA can be efficiently inhibited by LipofectamineTM2000
with HIF-1? siRNA through intravitreal injection in the diabetic rats. New gene
therapy may provided to control new vessels of diabetic retinopathy.
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FP-RET-FR 60 (8)
Lutein and Omega-3-Fatty Acids Supplementation in AMD Patients
The LUTEGA-Study Two Year Results in a Crossover Design
Dawczynski Jens (1) , Jentsch Susanne (2) , Schweitzer Dietrich (2) , Hammer
Martin (2) , Lang Gabriele (3)
1. University Hospital Leipzig, Department of Ophthalmology
2. University Hospital Jena, Department of Ophthalmology
3. University Hospital Ulm, Department of Ophthalmology
Effect of a supplementation with lutein (L), zeaxanthin (Z), and omega-3-fattyacids
(O-3-FA) on optical density of macular pigment (MPOD) and visual acuity
was investigated in AMD patients. MPOD (max OD, mean OD, volume, area)
was measured using the 1-wavelength reflection method recording reflection
images at 480 nm. Study population was divided into two treatment arms
(D1: 10mg L, 1mg Z, 255mg O-3-FA; n=16/ D2: 20mg L, 2mg Z, 510mg O-3-
FA, n=20). After 12 months supplementation was changed in a crossover
design and patients were supplemented for additional 12 months. All MPOD
parameters increased during second year of supplementation in the D1?D2
group, while volume, mean OD and max OD slightly decrease during the
second year in the D2?D1 group. Visual acuity further increased in both groups
within the second year of supplementation (D1?D2 after 2 years +3.37 letters;
D2?D1 after 2 years +4.04 letters). During the second year of supplementation
a further stabilization of MPOD was seen. A crossover to D2 resulted in an
additional increase of MPOD. Visual acuity further increased in all groups
during the second year of supplementation. Additional increase of visual acuity
during the second year of supplementation was higher in the D2?D1 group
compared to D1 D2.
FP-RET-FR 60 (9)
Biocompatibility of BBG Depends on Low Cellular Permeability into
the Live Cells.
Hisatomi Toshio (1,2) , Notomi Shoji (1) , Enaida Hiroshi (1) , Ishibashi Tatsuro (1)
1. Department of Ophthalmology, Kyushu University
2. Kyushu Medical Center Hospital
Purpose: We have reported Brilliant Blue G as a surgical adjuvant with good
biocompatibility and staining ability of internal limiting membrane (ILM). To
examine the biocompatibility of BBG, we evaluate intracellular intake and
accumulation of the dye. Since recently BBG was reported to be an antagonist
of P2X7 receptor, we tested possible contribution of P2X7 receptor on the dye.
Methods: The mouse primary retinal cell culture was tested under normal and
starvation condition. Intracellular accumulation of Calsein AM and CMTMRos
was analyzed for live cell imaging while propidium iodide for dead cell imaging.
TUNEL was also stained for apoptotic cell death. To test the role of P2X7
receptor, P2X7 knockout mouse was also compared to wild type mouse.
Results: Apoptotic cell death was increased under starvation condition. The
live cells barely stained with BBG. A part of TUNEL positive apoptotic cells and
PI positive necrotic cells was positive for BBG. P2X7 receptor knockout mouse
showed no remarkable changes on BBG staining.
Conclusion: The live cells showed no staining of BBG, confirming substantial
biocompatibility of the dye. The staining of apoptotic and necrotic cells was
dependent on the cellular permeabilization after cell death rather than P2X7
receptor.