Table of Contents - WOC 2012

WOC2012 Abstract Book
FP-COR-FR 97 (4)
Ultra-Structural Changes in Hydrated Camel Corneal Stroma
Akhtar Saeed (1) , Khan Mohmmed FJ (1) , Ahmed Merajuddin (1)
1. Cornea Research, OPTOM, CAMS, King Saud University
Purpose: Previous studies have shown the swelling characteristics of camel
cornea. Here we investigate the changes in the architecture of corneal stroma
due to hydration.
Method: Six fresh camel corneal buttons (8.5mm) were hydrated separately in
deionised water for 24hrs and 48hrs. The change in hydration of corneal
stroma was calculated per unit weight. Three corneas of each hydrated for
24hrs and 48 hrs were fixed in 2.5% glutaraldehyde containing cuprolinic blue
in sodium acetate buffer and processed for electron microscopy.
Result: The hydration of corneal stroma after 24hrs and 48hrs was 54 per unit
weight and 56 per unit weight respectively. Hydration had a minor affect on the
anterior stroma but had a severe affect on middle and posterior stroma. The
collagen fibrils were randomly distributed and had large interfibrillar spacing in
between them especially in the middle and posterior stroma. There was large
PG filaments present just above the Descemet’s membrane.
Conclusion: Our studies showed that camel cornea has very high hydration
during the first 24 hrs. After 24hrs, the hydration is very low. The hydration
of the cornea severely affected the uniform distribution of collagen fibrils and
proteoglycans.
FP-COR-FR 97 (5)
TGFBI Gene Mutation in a Chinese Pedigree with Reis-Bucklers
Corneal Dystrophy
Liang Qing-feng (1) , JIN Xiu-ying (1) , Deng Shi-jing (1) , SUN Xu-guang (1)
1. Beijing Institute of Ophthalmology, Beijing Tongren Eye Center
Purpose: To characterize the molecular defects in the TGFBI gene in a Chinese
family with Reis-Bücklers corneal dystrophy (RBCD), and to study the
relationship between the gene mutation, the clinical manifestations and
histopathological appearance.
Methods: Four generations of the family with RBCD were enrolled in the
present study. Ophthalmologic and pathological examinations, PCR
amplification and nucleotide sequencing of exons of TGFBI were performed.
Results: The clinical features of the disease were characterized by geographic
opacities in subepithelial layers and anterior stroma of the cornea. Confocal
microscopy images showed focal hypo-reflective materials deposited in the
subepithelium and anterior stroma. It was confirmed by histopathology that
Bowman›s membrane was mainly replaced by a moderately cellular, fibrillar
material, which extended inwards into the superficial corneal stroma. Molecular
genetic analysis revealed a single heterozygous G>T at nucleotide 124 in exon
4 of TGFBI in all members (twenty-two) of the pedigree affected with RBCD.
Conclusions: R124L mutation of the TGFBI gene is found in this biggest
Chinese family pedigree with Reis-Bücklers corneal dystrophy. The phenotype
in this family belongs to the geographic type. Molecular genetics approach
combination with histopathology may be useful for the proper diagnosis of this
type of corneal dystrophy.
124
FP-COR-FR 97 (6)
Is Microsporidial Keratitis an Emerging Cause of Infective Keratitis?
A Case Series Study
Bacchav Ashish (1) , R Ramakrishana (1) , Reddy Venkat (1) , Raut Shalmali (1)
1. Aravind Eye Hospital
Background: Microsporidial keratitis is a rare cause of infective keratitis. We
present a series of three cases of microsporidial keratitis from a single centre
in southern India with microbiologic and histopathologic features.
Case presentation: Patient charts of three cases of microsporidial keratitis
diagnosed between January 210 and June 2011 were reviewed retrospectively
for clinical data, microbiologic and histopathologic data. The presence of
microsporidia was confirmed by special stains on corneal scrapings and/or
corneal tissues, and electron microscopy. All patients were immunocompetent.
Two patients presented with unilateral, small, persisting epithelial and
subepithelial infiltrates, in the cornea, which were not responding to conventional
antimicrobial treatment. One case was unsuspected and not responding to
maximum possible antimicrobial therapy and underwent keratoplasty. The
microsporidia appeared as oval well defined bodies with dense staining at one
pole. No recurrence was seen following keratoplasty.
Conclusion: Microsporidia, though rare, should be suspected in chronic
culture-negative infective keratitis. Organisms could lie dormant without
associated inflammation.
FP-COR-FR 97 (7)
Complications of Collagen Crosslinking Procedure (CXL) for
Progressive Keratoconus (KCN) and Post LASIK Keratectasia.
Sejpal Kunjal (1) , Taneja Mukesh (2) , Murthy Somasheila (2) , Krishna Pravin (2)
1. Comprehensive and Cornea Services, L V Prasad Eye Institute
2. Cornea and Laser Refractive Surgery services, L V Prasad Eye Institute
Purpose: To evaluate the complications after collagen crosslinking procedure
for keratoconus and post LASIK keratectasia and their outcomes.
Methods: Retrospective chart review of all cases of collagen crosslinking
performed between August 2008 and May 2011 at a tertiary care institute.
Results: 101 eyes underwent collagen crosslinking, of which complications
were noted in 11 eyes (10.8%) at a mean period of 6 days after the procedure.
Five eyes developed sterile infiltrates which responded to topical steroids. In
4 eyes, focal or diffuse corneal edema with/without cellular deposits on the
endothelium was noted. Edema resolved with topical steroids. 1 eye developed
HSV epithelial keratitis and was treated with antivirals. Infectious keratitis with
gram positive cocci presenting as multiple peripheral infiltrates was noted in 1
eye and was treated with antimicrobials. Visual acuity was not affected in any
eye. Stromal haze, though common, was not visually limiting.
Conclusions: Collagen crosslinking is a relatively safe procedure. Complications
are mostly contact-lens related. Transient endothelial insult may occur. Close
follow-up in the postoperative period is warranted.