Table of Contents - WOC 2012

PO-COR-65
Tpical tacrolimus as a treatment for sterile corneal infiltrates in
psoriasis
González-Andrades Miguel (1) , Pérez-Roca Fernando (2) , González-Gallardo
Carmen (1) , García-Serrano Jose Luis (1) , Rodrigo Morales Esther (1)
1. University Hospital San Cecilio.
2. University Hospital Virgen de las Nieves.
Objectives & Purpose: Psoriasis is a common chronic inflammatory skin
disease in which corneal participation is infrequent. In the present study,
topical tacrolimus is evaluated as a treatment for sterile corneal infiltrates in
the context of psoriasis disease.
Methods: Two different patients presented to the emergency room complaining
about red and pain eye which did not heal after topical applications of antibiotic
ointments, coinciding with a cutaneous psoriasis outbreak. Biomicroscopy
showed conjunctival hyperaemia, superior pannus and marginal sterile corneal
infiltrates (corneal cultures were negative) that did not disappear after applying
topical 1 mg/ml dexamethasone (b.i.d.) during one week. Finally, 0.03%
tacrolimus pomade (b.i.d.) alone was applied during 30 + 14 days.
Results: Topical tacrolimus treatment made corneal infiltrates disappear
achieving very successful results with no recurrence after nine-month followup.
Conclusions: There are other available treatments such as immunosuppressive
drugs, which could represent an alternative to the conventional treatment
applied. In this study, 0.02% topical tacrolimus ointment has been applied to
both patients achieving successful results.
PO-COR-66
Effect of Long Term Topical Administration of Diclophenac Sodium
on Rats Cornea Studied by Light and Electron Microscopy
Osman Ahmed (1) , Farghaly Eman (2)
1. Alexandria Faculty of Medicine, Ophthalmology Department
2. Alexandria Faculty of Medicine,Histology and Cell Biology Department
Objective: There are many side effects of several drugs on ocular tissues both
used to treat diseases of different body organs or to treat eye problems .
Diclofenac Sodium is used in the treatment of many diseases and infections
of the eye but no histological study of the side effects on ocular tissues was
carried out. This research was designed to study side effects of sodium
diclofenac on the cornea of white mice .
Methods: The sample included was divided up into two groups . Na Diclofenac
drops was applied four times daily for 8 weaks to mice of the first group and
the second group was used as a control one with placebo application. Animals
were sacrificed and corneas were prepared for the study. Results showed
that there are Histological & Ultrastructural changes: as epithelial thickening.,
Intracellular oedema.,Stromal oedema. And Endothelial damage of several
degrees.
WOC2012 Abstract Book
PO-COR-67
Risk factor of the corneal epithelial disorders after vitreoretinal
surgeries
Deguchi Yuzo (1) , Hori Yuichi (1) , Maeno Takatoshi (1)
1. Department of Ophthalmology, Toho University Sakura Medical Center
Purpose: To determine factors associated with development of corneal
epithelial disorders after vitreoretinal surgeries.
Methods: The records of 952 patients who had undergone a vitreoretinal
surgery at the Toho University Sakura hospital, were reviewed retrospectively;
and age, gender, surgical time, history of diabetes mellitus (DM), intraoperative
corneal epithelial débridement, development of corneal epithelial disorders and
gas or silicon oil tamponade postoperatively were recorded. Multiple regression
analysis was performed of the factors associated with the development of
corneal epithelial disorders after vitreoretinal surgeries.
Result: The incidence of a postoperative corneal epithelial disorder was 25.0%.
Because multivariate analysis showed intraoperative corneal epithelial
débridement has an enormous effect on the development of a corneal
epithelium disorder, we excluded 170 cases in which corneal débridement
was performed intraoperatively. After the exclusion, the incidence of that was
significantly higher in patients with DM (10.9%) than without DM (6.7%, p =
0.04). Multiple regression analysis showed that the presence of DM and a
surgical time exceeding 90 minutes are linked significantly to the development
of a corneal epithelium disorder after vitreoretinal surgeries.
Conclusion: This retrospective study indicated that DM and a long surgical time
are associated with the onset of a postoperative corneal epithelium disorder
PO-COR-68
Gene Expression Analysis of Conjunctival Epithelium before and
after Diquafosol Sodium Eye Drop
Kawakita Tetsuya (1) , Kato Hisanori (2) , Shimmura Shigeto (1) , Tsubota Kazuo (1)
1. Department of Ophthalmology, Keio University School Of Medicine
2. Graduate school of agricultural and life science, The University of Tokyo
Objective: Diquafosol sodium eye drop has been reported to regulate mucin
expression in conjunctiva. To analyze the gene which is upregulated and
downregulated before and after 3% diquafosol sodium eye drop instillation in
human.
Methods: Conjunctival epithelial samples (upper bulbar conjunctiva) were
obtained from healthy volunteers (n=4) by impression cytology before and 10
min after 3% diquafosol sodium eye drop instillation. Conjunctival epithelial
samples were used for microarray gene expression analysis and PAS staining.
For microarray, enough RNA was purified from the sample, and the comparison
analysis was performed in obtained data.
Results: In comparison analysis, 31 genes were upregulated, and 17 genes
were downregulated. Among them, membrane-type mucin, MUC1,MUC4,
Muc15 was not significant difference before and after diquafosol sodium eye
drop instillation, however MUC5AC was upregulated after eye drops, which
was supported by the increase of PAS staining after eye drop.
Conclusion: MUC5AC gene was upregulated with more gel-type mucin
expression after one time Diquafosol sodium eye drop.
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