Table of Contents - WOC 2012

WOC2012 Abstract Book
PO-ONC-10
Clinical Features of Retinocytoma: A Case series
Chawla Bhavna (1) , Jain Amit (1)
1. All India Institute of Medical Sciences
Objective: To report the clinical features of retinocytoma, a benign variant of
retinoblastoma (Rb).
Methods: Retinocytoma was diagnosed as an incidental finding in 3 cases who
presented to our centre between 2010-2011
Results: In Case 1 (27 yrs, F) and Case 2 (30 yrs, F), the tumor was detected
on parental screening, as both cases presented with a child affected
with bilateral Rb. Case 3 was a 7 yr old male with a history of enucleation
elsewhere for Rb, and the retinocytoma was discovered in the other eye on
routine examination. The right eye was affected in all cases and the tumor was
extramacular in location. Visual acuity in the affected eye was 6/9 in Case 1 &
3, and 2/60 in Case 2. Ophthalmoscopic features included translucent retinal
mass, chorioretinal atrophy, and calcification. Co-existent iridofundal coloboma
was noted in Case 2. Transpupillary Thermotherapy was performed in Case 3.
Conclusion: Though a rare tumor, awareness regarding clinical features and
natural history of retinocytoma and its differentiation from Rb is important.
Meticulous follow-up is warranted in a case of retinocytoma, as it may rarely
undergo malignant transformation
PO-ONC-11
Role of 18-F Fluorodeoxyglucose Positron Emission Tomography/
Computed Tomography (PET/CT) scan in choroidal melanoma: a
pilot study on Indian eyes
Chawla Bhavna (1) , Kumar Rakesh (1) , Gupta Anoop (1) , Sahoo Manas (1) , Sen
Seema (1)
1. All India Institute of Medical Sciences
Purpose: To evaluate the role of 18- F fluorodeoxyglucose (FDG) Positron
Emission Tomography/Computed Tomography (PET/CT) in cases of advanced
choroidal melanoma
Methods: Patients with a clinical diagnosis of advanced choroidal melanoma
who presented to our center between 2009-2011 underwent 18F-FDG PET/
CT scan prior to enucleation for the detection of primary tumor as well as
distant metastasis. Intravenous injection of radiotracer 370 MBq of 18F-FDG
was given and 60 minutes later, PET-CT images were acquired in a craniocaudal
fashion on a PET/CT scanner. Results of imaging were analysed by two
experienced nuclear medicine physicians.
Results: Twenty patients (13 males, 7 females) were evaluated. All cases
(100%) showed abnormal tracer uptake in the choroidal region of the eye.
Although routine metastatic work-up was within normal limits in all cases, 2
patients (10%) had abnormal radiotracer uptake in distant locations on PET/
CT scan.
Conclusion: PET/CT scan is emerging as an invaluable imaging modality in
patients with choroidal melanoma, as a single test which can be used for the
detection and localization of primary tumor as well as for metastatic disease.
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PO-ONC-12
Single Nucleotide Polymorphism (SNP) based pyrosequencing for
detection of chromosome 3 monosomy on fine needle aspiration
biopsies (FNABs) of uveal melanoma patients
Einbock Wilma (1) , Siebolts Udo (2) , Wiedemann Peter (1) , Wickenhauser C. (2) ,
Wittekind Christian (2)
1. University Hospital of Leipzig, Department of Ophthalmology
2. University Hospital of Leipzig, Institute of Pathology
Purpose: About 50% of uveal melanoma carry chromosome 3 monosomy,
which has been shown to be a significant predictor of metastatic disease and
poor prognosis.
Methods: From 15 uveal melanoma patients H&E staining was performed on
FNA smears for determination of melanoma subtype. DNA was extracted from
the smears and from blood of the respective patients. Blood DNA was screened
for informative because heterozygous SNP loci on chromosome 3 performing
pyrosequencing. Accordingly, data were compared with those obtained by
tumour DNA analysis. In case of pseudo-homozygous conversion of the tested
SNP, allele-loss and therefore monosomy was stated. Results were verified by
fluorescence in situ hybridisation (FISH) on smears and corresponding tissue
probes. In addition, pyrosequencing was performed on histomorphologic
evaluated, macro dissected tumour tissues of the enucleated eyes.
Results: In all cases under study FNA smears permitted accurate classification
of melanoma subtype when compared to the enucleates. Furthermore,
subsequent DNA extraction allowed pyrosequencing with accordance of 100%.
Conclusion: We established a reliable, easy to use method on FNA with first,
evaluation of the histological subtype and second, subsequent DNA extraction
and testing for monosomy 3.
PO-ONC-13
A CLINICAL AND HISTOPATHOLOGICAL CORRELATION OF EYE LID
TUMORS
Pai Vijaya (1) , Pai Prathvi (1) , Hazarika Manali
1. Dept of Ophthalmology,Kasturba Medical College, Manipal University,
OBJECTIVE: A clinical and histopathological correlation of eyelid tumours
Methods: A retrospective analysis of eyelid tumours between 2000 and 2011 .
Results: 11 patients (7-male, 4-female) were included. Age of the patients
varied from 7 to 82 years (Mean-58.9). Duration of illness ranged from one
year to 12 years. Clinical diagnosis suspected were basal cell carcinoma-6,
squamous cell carcinoma (SCC)-3, molluscum contagiosum/papilloma-1 and
keratoacanthoma-1. The histopathology of tumour correlated with clinical
diagnosis in three patients (i.e., BCC). Other histopathological diagnosis being
SCC-1, malignant melanoma (MM) -1, Seborrhoeic keratoses-1, meibomian
gland carcinoma-1, sebaceous carcinoma-1, cutaneous myxoma-1, ocular
myiasis-1 and sebaceous papilloma-1. Orbital involvement was seen in 2
patients. 2 patients had lymph node involvement . 9 patients underwent wide
mass excision and lid reconstruction and 2 patient underwent exentration (BCC
and Orbital myiasis).Additional radiotherapy was given in 3 patients (BCC-1,
meibomian gland carcinoma-1, sebaceous carcinoma-1). Chemoradiation
was done in one patient (MM). Recurrence noted in 1 patient with SCC during
follow up period.
Conclusion: This study Gives perspective of the myriad presentation of tumours
of the eyelids. Histopathological and clinical correlation was noted in only three
cases. This may be in view of the rarity of tumours.
PO-ONC-14
Complete cheek basal cell carcinoma remission with imiquimod in a
patient with contralateral lower lid basal cell carcinoma excision
Perez Benjamin (1,2) , Rivera Jorge (1,2) , Navarro Patricia (1,2)
1. Centro Especializado en Oncologia Ocular
2. Centro Ocular Texcoco
Objective/Purpose To report the succesfully treatment of basal cell carcinoma
of the cheek with topical imiquamod - Methods A 75 year-old female patient
who previously underwent surgical excision of basal cell carcinoma of the left
lower lid was treated with topical imiquamod four times a day for six weeks
on a right cheek lesion - Results There were complete resolution of the cheek
lesion after six weeks of topical imiquamod - Conclusion Imiquamod is a good
treatment option for basal cell carcinoma of the lid skin and sourrounding areas