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information of consumers of freshwater fish in the catchment, bioaccumulation<br />

potential and health risks of analytes, sound sampling design, risk <strong>as</strong>sessment<br />

procedures and performing monitoring at different scales and depth (Figure 1). It is<br />

important to note the method developed is closely linked to the protocols proposed<br />

by the USEPA (1995a,b, 1996, 1997) for issuing fish consumption advisories for noncommercial<br />

fish and by Heath (1999) for the monitoring of pesticides and metals in<br />

South African rivers. The approach by Heath (1999) and the current approach are<br />

catchment-b<strong>as</strong>ed, making it possible to use much the data and information when<br />

undertaking any of the proposed levels of investigation. Therefore, if projects are<br />

carefully planned using the same methodology and principles, the data and information<br />

can be exchanged, which would ensure the optimal utilisation of resources.<br />

South African Methodology<br />

The methodology developed for South African human health risks <strong>as</strong>sociated with<br />

eating freshwater fish identifies ten major steps, namely:<br />

(i) selection of scale and depth of survey,<br />

(ii) <strong>as</strong>sessment of the water-body catchment,<br />

(iii) monitoring system design,<br />

(iv) field collection,<br />

(v) laboratory sample processing and analysis,<br />

(vi) analysis of and reporting of results,<br />

(vii) risk <strong>as</strong>sessment,<br />

(viii) risk management,<br />

(ix) risk communication, and<br />

(x) evaluation and review of the programme.<br />

The application of the protocol developed, even though it h<strong>as</strong> several sequential<br />

steps, must be seen <strong>as</strong> an iterative process. For example, during the <strong>as</strong>sessment of<br />

the water body (Step 2) it is important understand the needs of the risk <strong>as</strong>sessment<br />

(Step 7) so that the relevant information is collected at the appropriate time. The<br />

same applies to the collection of sample in the field (Step 4), which need to be<br />

collected and preserved according to the analytical Procedures (Step 6).<br />

The b<strong>as</strong>ic requirements of each step is highlighted <strong>as</strong> limited resources (financial,<br />

infr<strong>as</strong>tructure and skilled personnel) in South Africa would limit the possibility of<br />

undertaking detailed <strong>as</strong>sessments <strong>as</strong> undertaken by the United States of America<br />

Environmental Protection Agency (USEPA). Nevertheless, by applying the proposed<br />

protocol, sound comparable <strong>as</strong>sessments, b<strong>as</strong>ed on risk <strong>as</strong>sessment methodology,<br />

can be made regarding the human health risk <strong>as</strong>sociated with the consumption of<br />

freshwater fish in South Africa.<br />

Three monitoring levels are identified for the investigation of the chemical contaminant<br />

concentrations in freshwater fish tissue (Figure 1). The following levels of monitoring<br />

are considered for the South African surveys, namely Screening surveys, Intensive<br />

surveys (Ph<strong>as</strong>e I) and Intensive surveys (Ph<strong>as</strong>e II). The intensity of the level of<br />

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