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Table 2:
Regression functions and correlation coefficients of the studied
pharmaceuticals
Pharmaceutical Regression function R 2 Linearity
range
(ng/spot)
LOD (ng/
spot)
LOQ
(ng/
spot)
NOR A = 2.286 . 10 6 . + 930.8 0.994 0.75–7.5 0.5 0.75
OTC A = 29.6400 . - 429.3 0.994 50–250 25 50
TMP A = 6.3470 . - 6.821 0.997 75–300 50 75
SDIAZ A = 38.8400 . + 1445.0 0.998 50–200 25 50
SMETH A = 26.9500 . + 1496.0 0.999 50–200 25 50
PGP A = 18.300 . + 1090.8 0.993 50–200 25 50
SGUA A = 15.7500 . + 3265.0 0.996 150–550 100 150
A-peak area.
g-mass concentration (ng/spot) (spot = 5 μL).
Precision expressed in terms of RSD indicated high measurement repeatability (Table
3). In general, recoveries were dependent on the analyte concentration and values
between 36.5% and 100.5% were obtained for all pharmaceuticals.
Table 3:
Recoveries and RSDs obtained for studied pharmaceuticals
Pharmaceutical Recovery (%) RSD (n = 3)
NOR 36.5 4.05
OTC n.d. 2.86
TMP 52.6 9.83
SDIAZ 83.2 6.00
SMETH 100.5 6.50
PGP 44.7 3.12
SGUA 78.5 4.88
ND: not detected.
According to validation parameters, it can be concluded that USE-TLC determination
of veterinary antibiotics in soil is comparable with other commonly used extraction
and chromatographic techniques, and convenient for determination of veterinary
pollutants in soil (Thiele-Bruhn, 2003).
ACKNOWLEDGEMENTS
This work has been supported by: EU project EMCO (INCO CT 2004-509188)
- Reduction of environmental risks, posed by Emerging Contaminants, through
advanced treatment of municipal and industrial wastes and Croatian Ministry of
Education, Science and Sport (Project No. 0125055).
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