Liquid Culture Systems for in vitro Plant Propagation

158 Annette Hohe & Ralf Reski
dry weight [mg/l]
dry weight [mg/l]
dry weight [mg/l]
800
700
600
500
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200
100
0
2.0
0 1 2 3 4 5 6 7 8 9 10 11
800
700
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A
time [d]
dry weight, aeration with 2 % CO2 dry weight, aeration with air
pH, aeration with 2 % CO2 pH, aeration with air
0
2.0
0 1 2 3 4 5 6 7 8 9 10 11
0
2.0
0 1 2 3 4 5 6 7 8 9
B
time [d]
dry weight, 24 h light dry weight, 16 h light
pH, 24 h light pH, 16 h light
C
time [d]
dry weight, 24 h light + 2 % CO2 dry weight, 24 h light dry weight, 16 h light
pH, 24 h light + 2 % CO2 pH, 24 h light pH, 16 h light
Figure 1: Effect of CO2 supplementation of the aeration gas and continuous illumination on
growth of photoautotrophically growing Physcomitrella in bioreactor batch cultures in
ammonium-supplemented medium. A: comparison of aeration with air and air supplemented
with 2 % (v/v) CO2. B: comparison of growth in photoperiods of 16 and 24 h. C: growth
curve obtained in culture aerated with CO2-enriched air and in continuous illumination.
Growth curves in one figure were obtained by running parallel bioreactor cultures using
identical inoculum.
n=2
n=2
6.0
5.5
5.0
4.5
4.0
3.5
3.0
2.5
6.0
5.5
5.0
4.5
4.0
3.5
3.0
2.5
6.0
5.5
5.0
4.5
4.0
3.5
3.0
2.5
pH
pH
pH