Liquid Culture Systems for in vitro Plant Propagation

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Micropropagation of Rosa 381 The rooted microshoots (Figure 3c-d) were incubated in rooting vessel for different times before their transfer to greenhouse conditions. It was observed that highest survival percentage (96.7% in R. damascena and 100% in R. bourboniana) was recorded after 6 weeks of incubation (Table 3 and 4). However, at this stage, most of the shoots were touching the top of the box (Figure 3e) and therefore, required immediate transplantation. These plantlets were kept in a greenhouse for 6-8 weeks before their transfer to larger pots (20cm) (Figure 3f) and eventually, to the fields. 3.3 Comparative response of micropropagation of R. damascena and R. bourboniana A comparative analysis of different stages of micropropagation of Rosa damascena and R. bourboniana indicates that these two species did not have much difference in respect to different test parameters under the in vitro culture systems tested (Table 4). Table 1: Development of cultured shoots of R. damascena in agar-gelled and liquid MS+BAP (5µmol sucrose (3% (w/v)) Medium Gelled (Agar: 0.8 % w/v)) Liquid No. of shoots Initial Final* 7.2 ± 0.7 7.2 ± 0.37 30.4 ± 4.1 58.0 ± 2.60 * Data recorded after 6 weeks of culture Shoot length (cm) Initial Final* 1.08 ± 0.03 1.04 ± 0.02 1.62 ± 0.03 2.96 ± 0.2 Stem thickness (mm) Initial Final* 0.90 ± 0.04 0.92 ± 0.1 Table 2: Comparison of rooting response* in liquid and agar-gelled media** 1.54 ± 0.1 1.96 ± 0.05 Liquid medium Agar-gelled medium Time to root induction 8 days 10 days Rooting (%) 85.8 5% Root length (mm) 3.5-4.0 1.5-2.5 Root number per shoot 8-10 3-6 *Data recorded after 3weeks **1/2 MS+ IBA (10.0 �mol) + sucrose (3% (w/v)) for 1week, then transfer to 1/2 strength MS without PGRs
382 Pratap Kumar Pati et al. Table 3: Effect of R. damascena microshoot incubation period in rooting vessels, on the percent survival after transfer to greenhouse Week of transfer Survival (%) 1 3.3±3.3 2 23.3±3.3 3 60.0±5.7 4 66.7±8.8 5 73.3±8.8 6 96.7±3.3 Table 4: Comparative response of R. damascena and R. bourboniana to micropropagation protocols Stage of micropropagation * Initiation of aseptic culture ** Shoot proliferation *** Rooting **** Hardening and plantlet establishment Parameters R. damascena Contamination rate Response R. bourboniana 29.6 � 1.9 18.6 � 3.8 Proliferation rate (No. of shoots per explant) 8.1 ± 0.2 7.5 � 0.2 Root induction (days) 7-8 7-8 Rooting (%) 85.8 � 0.6 94.9 � 2.1 Root length (mm) 4.6 � 0.4 5.2 � 0.4 Root number per shoot 9.0 � 0.5 11.4 � 0.4 Survival (%) 96.7 100 Remarks Surface of nodal explants of R. bourboniana was smooth and had fewer / smaller thorns compared to R. damascena R. bourboniana shoots were sturdy, leaves dark green with greater surface area compared to R. damascena Rooting is easier in R. bourboniana. Basal MS medium produces rooting in R. bourboniana unlike R. damascena Hardening is easier in R. bourboniana compared to R. damascena *Data recorded after 4 weeks of culture in MS medium without PGRs **Data recorded after 6 weeks of culture in liquid MS medium + BAP (5 µmol) + sucrose (3% (w/v)) ***Data recorded after 3 weeks of culture; 1 week in liquid ½ MS + IBA (10 µmol) + sucrose (3% (w/v)) and 2 weeks in MS + sucrose (3% (w/v)) ****Data recorded after 3 weeks of transplantation in potting mix (sand : garden soil, 1: 1)
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LIQUID CULTURE SYSTEMS FOR IN VITRO
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A C.I.P. Catalogue record for this
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Contents Contributing Authors xiii
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Liquid culture systems for in vitro
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Contributing Authors Adelberg, J. 4
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Preface Plant cell, tissue and orga
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Chapter 1 General introduction: a p
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General introduction 3 2. Cell susp
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General introduction 5 rooted in gr
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General introduction 7 (Winkelmann
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General introduction 9 the bioreact
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General introduction 11 (TIS), resu
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General introduction 13 Barz W, Rei
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General introduction 15 Hohe A, Win
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General introduction 17 Shimazu T &
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I. Bioreactors
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22 Wayne R. Curtis 1. Introduction
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24 Wayne R. Curtis headspace double
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26 Wayne R. Curtis that is required
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28 Wayne R. Curtis C L y � P O2
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30 Wayne R. Curtis since there is a
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32 Wayne R. Curtis 5 cm 30 cm 30 o
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34 Wayne R. Curtis Pˆ N � � me
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36 Wayne R. Curtis Radial Flow Impe
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38 Wayne R. Curtis CS = Medium diss
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40 Wayne R. Curtis Murashige T & Sk
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42 Anne Kathrine Hvoslef-Eide et al
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Chapter 4 Practical aspects of bior
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Practical aspects of bioreactor app
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Chapter 5 Simple bioreactors for ma
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Simple bioreactors for mass propaga
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96 K. Y. Paek et al. opportunity fo
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98 K. Y. Paek et al. disadvantages
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100 K. Y. Paek et al. 3.1 Dissolved
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102 K. Y. Paek et al. optimizing bi
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104 K. Y. Paek et al. cosmetics, wh
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106 K. Y. Paek et al. protocol, mor
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108 K. Y. Paek et al. use. In order
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110 K. Y. Paek et al. a b c d e f F
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112 K. Y. Paek et al. a b c d e f F
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114 K. Y. Paek et al. Escalona M, L
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116 K. Y. Paek et al. Son SH & Paek
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118 Seppo Sorvari et al. economical
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120 Seppo Sorvari et al. membrane w
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122 Seppo Sorvari et al. 3. Results
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124 Seppo Sorvari et al. D.O. 160 1
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Chapter 8 Cost-effective mass cloni
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Cost-effective mass cloning of plan
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144 Eun-Joo Hahn & Kee-Yoeup Paek 1
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146 Eun-Joo Hahn & Kee-Yoeup Paek F
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148 Eun-Joo Hahn & Kee-Yoeup Paek T
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150 Eun-Joo Hahn & Kee-Yoeup Paek 3
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152 Eun-Joo Hahn & Kee-Yoeup Paek E
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Chapter 10 Control of growth and di
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Control of growth and differentiati
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Chapter 11 Temporary immersion syst
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Temporary immersion system: a new c
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198 Elio Jiménez González 1. Intr
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200 Elio Jiménez González Several
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202 Elio Jiménez González Figure
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204 Elio Jiménez González exploit
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206 Elio Jiménez González weeks i
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208 Elio Jiménez González germina
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210 Elio Jiménez González Escalan
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Chapter 13 Use of growth retardants
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Use of growth retardants for banana
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Chapter 14 Somatic embryo germinati
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Somatic embryo germination of Psidi
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Somatic embryo germination of Psidi
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232 Tino Hempfling & Walter Preil N
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234 Tino Hempfling & Walter Preil 3
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236 Tino Hempfling & Walter Preil F
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238 Tino Hempfling & Walter Preil F
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240 Tino Hempfling & Walter Preil 4
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242 Tino Hempfling & Walter Preil R
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244 C. Damiano et al. Over the last
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246 C. Damiano et al. 2.2 Temporary
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248 C. Damiano et al. Table 3: Wate
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250 C. Damiano et al. hyperhydricit
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Chapter 17 Optimisation of growing
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Optimisation of growing conditions
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264 Katerina Grigoriadou et al. cul
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266 Katerina Grigoriadou et al. 2.4
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268 Katerina Grigoriadou et al. mic
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270 Katerina Grigoriadou et al. of
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272 Katerina Grigoriadou et al. 4.
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274 Katerina Grigoriadou et al. Tei
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276 Ch. Wawrosch et al. possesses s
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278 Ch. Wawrosch et al. 3. Results
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280 Ch. Wawrosch et al. References
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Chapter 20 Propagation of Norway sp
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Propagation of Norway Spruce 285 2.
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Propagation of Norway Spruce 287 su
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Propagation of Norway Spruce 289 5.
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Propagation of Norway Spruce 291 bl
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Propagation of Norway Spruce 293 H
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296 M. Vágner et al. 1995). In liq
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298 M. Vágner et al. maturation pe
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300 M. Vágner et al. mature somati
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302 M. Vágner et al. Acknowledgeme
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304 Christopher Hunter & Lionel Lev
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314 Michel Petitprez et al. 1. Intr
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316 Michel Petitprez et al. 2.2 QTL
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318 Michel Petitprez et al. Figure
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320 Michel Petitprez et al. Table 1
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322 Michel Petitprez et al. Gentzbi
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324 F. Afreen et al. 1. Introductio
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326 F. Afreen et al. precotyledonar
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328 F. Afreen et al. Dry mass (mg/e
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Page 424 and 425: 426 B. McAlister et al. 1. Introduc
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434 B. McAlister et al. Figure 3: M
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436 B. McAlister et al. Average mul
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438 B. McAlister et al. Table 4: Co
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440 B. McAlister et al. Semi-solid
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442 B. McAlister et al. Escalona M,
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444 Jeffrey Adelberg including Host
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446 Jeffrey Adelberg BioSystems (Ho
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448 Jeffrey Adelberg more prolific
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450 Jeffrey Adelberg vessel of liqu
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452 Jeffrey Adelberg Figure 4: Labo
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454 Jeffrey Adelberg constituted a
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456 Jeffrey Adelberg Acknowledgemen
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Chapter 35 Aeration stress in plant
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Aeration stress in plant tissue cul
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476 Hans R. Gislerød et al. 1. Int
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478 Hans R. Gislerød et al. biorea
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480 Hans R. Gislerød et al. A low
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482 Hans R. Gislerød et al. Table
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484 Hans R. Gislerød et al. common
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486 Hans R. Gislerød et al. can be
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488 Hans R. Gislerød et al. 4.1 Li
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490 Hans R. Gislerød et al. 4.3 Ca
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492 Hans R. Gislerød et al. Morten
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494 Han Bouman & Annemiek Tiekstra
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V. Biomass for Secondary Metabolite
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510 E. Gontier et al. 1. Introducti
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512 E. Gontier et al. 2. Materials
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514 E. Gontier et al. developed dra
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516 E. Gontier et al. 3.3 Establish
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518 E. Gontier et al. Fresh weight
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520 E. Gontier et al. 3.5 Effect of
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522 E. Gontier et al. 3.6 Scale up
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524 E. Gontier et al. Lorenzo JC, G
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526 Dirk Wilken et al. 1. Introduct
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528 Dirk Wilken et al. running at 6
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530 Dirk Wilken et al. described a
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532 Dirk Wilken et al. packed cell
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534 Dirk Wilken et al. bioactive co
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536 Dirk Wilken et al. Fowler MW (1
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Chapter 40 Cultivation of root cult
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Cultivation of root cultures of Pan
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Chapter 41 Optimisation of Panax gi
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Optimisation of Panax ginseng liqui
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558 S. M. Nalawade et al. performan
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560 S. M. Nalawade et al. Plantlets
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562 S. M. Nalawade et al. leaves on
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564 S. M. Nalawade et al. roots (Fi
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Chapter 43 Production of taxanes in
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Production of taxanes 569 2. Materi
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Production of taxanes 571 Figure 2:
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Production of taxanes 573 various T
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Acknowledgments The editors thank t
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578 Contents 130, 131, 133, 134, 13
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580 Contents poplar, see Populus Po
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582 Contents bubble aerated 165 bub
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584 Contents ginsenoside content, p
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586 Contents plant growth regulator
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588 Contents -free microcorms 71 -f
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