Liquid Culture Systems for in vitro Plant Propagation

Experimental use of a novel temporary immersion system 269
a
Figure 3: Culture of olive microshoots in LifeReactor©: a) the system, b) sample of plant
material produced, c and d) callus formation on the surface of the shoots and leaves.
3.2 Liquid medium culture
b
c d
Culture in LifeReactor © resulted in 0.35 new microshoots per explant,
with an average length of 0.40 cm (Table 2). These small microshoots were
intensively hyperhydrized (macroscopically determined) and small callus
masses were present on the surface of the shoots and leaves (Figures 3b, 3c
and 3d).
Cultures on filter paper bridges formed 0.43 new microshoots per explant
with an average shoot length of 0.32 cm (Table 2). Extensive callus
formation was noticed at the base of the explants (Figure 4d).
Liquid culture of olive explants in Erlenmeyer flasks under agitation
resulted in the highest number of new microshoots per explant (1.93) with an
average shoot length of 0.40 cm (Table 2, Figure 4b). These microshoots
suffered from the most serious hyperhydricity compared to the other systems
tested.
The novel TIS proved to be quite effective as far as the development of
new microshoots in liquid media is concerned. The number of new
microshoots per explant was 1.93 and shoot length was 0.95 cm in the case