Liquid Culture Systems for in vitro Plant Propagation

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524 E. Gontier et al. Lorenzo JC, Gonzàlez BL, Escalona M, Teisson C, Espinosa P & Borroto C (1998) Sugarcane shoot formation in an improved temporary immersion system. Plant Cell, Tissue and Organ Culture 54: 197-200 Massot B, Milesi S, Gontier E, Bourgaud F & Guckert A (2000) Optimized culture conditions for the production of furanocoumarins by micropropagated shoots of Ruta graveolens. Plant Cell, Tissue and Organ Culture 62: 11-19 Milesi S, Massot B, Gontier E, Bourgaud F & Guckert A (2001) Ruta graveolens L.: a promising species for the production of furanocoumarins. Plant Science 161: 189-199 Milesi S, Massot B, Piutti S, Goergen JL, Murano E, Toffanin R, Bourgaud F, Gontier E & Guckert A (2000) Ruta graveolens cell cultures for psoralens production: culture in bioreactor, effect of elicitation and calcium alginate immobilization. International Journal of Biochromatography 5: 281-296 Pathak MA & Fitzpatrick TB (1992) The evolution of photochemotherapy with psoralens and UVA (PUVA): 2000 BC to 1992 AD. Journal of Photochemical and Photobiology B: Biology 14: 3-22
Chapter 39 Comparison of secondary plant metabolite production in cell suspension, callus culture and temporary immersion system Dirk Wilken 1* , Elio Jiménez González, Annette Hohe 1 , Miguel Jordan 3 , Rafael Gomez Kosky 2 , Guillermo Schmeda Hirschmann 4 & André Gerth 1 1 BioPlanta GmbH, Deutscher Platz 5, D-04103 Leipzig, Germany. E-mail: info@bioplanta- leipzig.de; 2 Instituto de Biotecnología de las Plantas, Carretera a Camajuaní Km. 5 ½, Santa Clara, Cuba. E-mail: ejimenez@uclv.edu.cu; rgkosky@uclv.edu.cu;mailto:rgkosky@uclv.edu.cu 3 Departamento de Ecología, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile. E-mail: mjordan@genes.bio.puc.cl; 4 Instituto de Quimica de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile. E-mail: schmeda@pehuenche.utalca.cl Abstract: Cell and organ cultures of Lavandula officinalis, Hypericum perforatum, Cymbopogon citratus and Fabiana imbricata were established for the production of secondary metabolites in vitro. Shoot multiplication was performed by conventional micropropagation on agar-solidified medium as well as in temporary immersion systems (TIS), the latter resulted in higher multiplication rates compared to the culture in microcontainers for all plant species tested. The concentration of bioactive compounds was determined in different in vitro cell and organ cultures and was compared to field grown plants. For Lavandula the highest content of rosmarinic acid was found in cell cultures, for the other three species in field grown plants. Concentrations of bioactive compounds were always higher in plant material grown in TIS compared to cell suspension and callus cultures. Key words: bioactive compounds, bioreactor culture, Cymbopogon, Fabiana, Hypericum, in vitro culture, Lavandula, pharmaceuticals, suspension culture Abbreviations: BA _ 6-benzylaminopurine; 2,4-D _ 2,4-dichlorophenoxyacetic acid; GC-MS _ mass spectroscopy combined with gas chromatography; HPLC _ high performance liquid chromatography; NAA _ �-naphtalene acetic acid; TIS _ temporary immersion system 525 A.K. Hvoslef-Eide and W. Preil (eds.), Liquid Culture Systems for in vitro Plant Propagation, 525–537. © 2005 Springer. Printed in the Netherlands.
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LIQUID CULTURE SYSTEMS FOR IN VITRO
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A C.I.P. Catalogue record for this
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Contents Contributing Authors xiii
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Liquid culture systems for in vitro
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Contributing Authors Adelberg, J. 4
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Preface Plant cell, tissue and orga
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Chapter 1 General introduction: a p
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General introduction 3 2. Cell susp
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General introduction 5 rooted in gr
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General introduction 7 (Winkelmann
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General introduction 9 the bioreact
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General introduction 11 (TIS), resu
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General introduction 13 Barz W, Rei
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General introduction 15 Hohe A, Win
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General introduction 17 Shimazu T &
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I. Bioreactors
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22 Wayne R. Curtis 1. Introduction
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24 Wayne R. Curtis headspace double
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26 Wayne R. Curtis that is required
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28 Wayne R. Curtis C L y � P O2
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30 Wayne R. Curtis since there is a
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32 Wayne R. Curtis 5 cm 30 cm 30 o
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34 Wayne R. Curtis Pˆ N � � me
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36 Wayne R. Curtis Radial Flow Impe
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38 Wayne R. Curtis CS = Medium diss
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40 Wayne R. Curtis Murashige T & Sk
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42 Anne Kathrine Hvoslef-Eide et al
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Chapter 4 Practical aspects of bior
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Practical aspects of bioreactor app
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Chapter 5 Simple bioreactors for ma
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Simple bioreactors for mass propaga
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96 K. Y. Paek et al. opportunity fo
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98 K. Y. Paek et al. disadvantages
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100 K. Y. Paek et al. 3.1 Dissolved
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102 K. Y. Paek et al. optimizing bi
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104 K. Y. Paek et al. cosmetics, wh
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106 K. Y. Paek et al. protocol, mor
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108 K. Y. Paek et al. use. In order
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110 K. Y. Paek et al. a b c d e f F
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112 K. Y. Paek et al. a b c d e f F
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114 K. Y. Paek et al. Escalona M, L
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116 K. Y. Paek et al. Son SH & Paek
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118 Seppo Sorvari et al. economical
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120 Seppo Sorvari et al. membrane w
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122 Seppo Sorvari et al. 3. Results
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124 Seppo Sorvari et al. D.O. 160 1
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Chapter 8 Cost-effective mass cloni
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Cost-effective mass cloning of plan
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144 Eun-Joo Hahn & Kee-Yoeup Paek 1
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146 Eun-Joo Hahn & Kee-Yoeup Paek F
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148 Eun-Joo Hahn & Kee-Yoeup Paek T
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150 Eun-Joo Hahn & Kee-Yoeup Paek 3
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152 Eun-Joo Hahn & Kee-Yoeup Paek E
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Chapter 10 Control of growth and di
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Control of growth and differentiati
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Chapter 11 Temporary immersion syst
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Temporary immersion system: a new c
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198 Elio Jiménez González 1. Intr
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200 Elio Jiménez González Several
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202 Elio Jiménez González Figure
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204 Elio Jiménez González exploit
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206 Elio Jiménez González weeks i
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208 Elio Jiménez González germina
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210 Elio Jiménez González Escalan
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Chapter 13 Use of growth retardants
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Use of growth retardants for banana
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Chapter 14 Somatic embryo germinati
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Somatic embryo germination of Psidi
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Somatic embryo germination of Psidi
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232 Tino Hempfling & Walter Preil N
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234 Tino Hempfling & Walter Preil 3
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236 Tino Hempfling & Walter Preil F
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238 Tino Hempfling & Walter Preil F
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240 Tino Hempfling & Walter Preil 4
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242 Tino Hempfling & Walter Preil R
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244 C. Damiano et al. Over the last
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246 C. Damiano et al. 2.2 Temporary
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248 C. Damiano et al. Table 3: Wate
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250 C. Damiano et al. hyperhydricit
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Chapter 17 Optimisation of growing
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Optimisation of growing conditions
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264 Katerina Grigoriadou et al. cul
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266 Katerina Grigoriadou et al. 2.4
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268 Katerina Grigoriadou et al. mic
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270 Katerina Grigoriadou et al. of
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272 Katerina Grigoriadou et al. 4.
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274 Katerina Grigoriadou et al. Tei
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276 Ch. Wawrosch et al. possesses s
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278 Ch. Wawrosch et al. 3. Results
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280 Ch. Wawrosch et al. References
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Chapter 20 Propagation of Norway sp
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Propagation of Norway Spruce 285 2.
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Propagation of Norway Spruce 287 su
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Propagation of Norway Spruce 289 5.
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Propagation of Norway Spruce 291 bl
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Propagation of Norway Spruce 293 H
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296 M. Vágner et al. 1995). In liq
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298 M. Vágner et al. maturation pe
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300 M. Vágner et al. mature somati
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302 M. Vágner et al. Acknowledgeme
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304 Christopher Hunter & Lionel Lev
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314 Michel Petitprez et al. 1. Intr
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316 Michel Petitprez et al. 2.2 QTL
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318 Michel Petitprez et al. Figure
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320 Michel Petitprez et al. Table 1
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322 Michel Petitprez et al. Gentzbi
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324 F. Afreen et al. 1. Introductio
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326 F. Afreen et al. precotyledonar
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328 F. Afreen et al. Dry mass (mg/e
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330 F. Afreen et al. under photoaut
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332 F. Afreen et al. Figure 3: a) C
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334 F. Afreen et al. the plant qual
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Chapter 25 Potential of flow cytome
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Potential of flow cytometry 339 dis
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Potential of flow cytometry 341 Fig
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Potential of flow cytometry 343 tha
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Chapter 26 Somatic embryogenesis of
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Somatic embryogenesis of Gentiana 3
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Chapter 27 Induction and growth of
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Induction and growth of tulip 361 a
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Induction and growth of tulip 363 T
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Chapter 28 Micropropagation of Ixia
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Micropropagation of Ixia 367 for PA
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Micropropagation of Ixia 369 Biomas
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Micropropagation of Ixia 371 Figure
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Chapter 29 Micropropagation of Rosa
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Micropropagation of Rosa 375 The BA
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Micropropagation of Rosa 377 Platfo
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Micropropagation of Rosa 379 3. Res
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Micropropagation of Rosa 381 The ro
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Micropropagation of Rosa 383 4. Dis
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Micropropagation of Rosa 385 Murash
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Chapter 30 Mass propagation of coni
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Mass propagation of conifer trees 3
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Chapter 31 Potentials for cost redu
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Potentials for cost reduction 405 c
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Potentials for cost reduction 407 c
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Potentials for cost reduction 409 s
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Potentials for cost reduction 411 N
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Potentials for cost reduction 413 G
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Chapter 32 A new approach for autom
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A new approach for automation 417 (
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A new approach for automation 419 p
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A new approach for automation 421 T
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A new approach for automation 423 D
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426 B. McAlister et al. 1. Introduc
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428 B. McAlister et al. 2.2 Multipl
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430 B. McAlister et al. Table 2: Mu
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432 B. McAlister et al. rest time -
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434 B. McAlister et al. Figure 3: M
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436 B. McAlister et al. Average mul
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438 B. McAlister et al. Table 4: Co
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440 B. McAlister et al. Semi-solid
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442 B. McAlister et al. Escalona M,
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444 Jeffrey Adelberg including Host
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446 Jeffrey Adelberg BioSystems (Ho
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448 Jeffrey Adelberg more prolific
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450 Jeffrey Adelberg vessel of liqu
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452 Jeffrey Adelberg Figure 4: Labo
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454 Jeffrey Adelberg constituted a
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456 Jeffrey Adelberg Acknowledgemen
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Chapter 35 Aeration stress in plant
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Aeration stress in plant tissue cul
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Page 468 and 469: Aeration stress in plant tissue cul
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Page 472 and 473: 476 Hans R. Gislerød et al. 1. Int
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Page 478 and 479: 482 Hans R. Gislerød et al. Table
Page 480 and 481: 484 Hans R. Gislerød et al. common
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Page 526 and 527: 532 Dirk Wilken et al. packed cell
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Page 542 and 543: Optimisation of Panax ginseng liqui
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Page 556 and 557: 564 S. M. Nalawade et al. roots (Fi
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Page 560 and 561: Production of taxanes 569 2. Materi
Page 562 and 563: Production of taxanes 571 Figure 2:
Page 564 and 565: Production of taxanes 573 various T
Page 566 and 567: Acknowledgments The editors thank t
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578 Contents 130, 131, 133, 134, 13
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580 Contents poplar, see Populus Po
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582 Contents bubble aerated 165 bub
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584 Contents ginsenoside content, p
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586 Contents plant growth regulator
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588 Contents -free microcorms 71 -f
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